5. Evaluation of acute and subchronic toxicity of An Nguyet Khang tablets in experimental animals
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The research evaluated the acute and subchronic toxicities of An Nguyet Khang tablets in experimental animals. Acute toxicity was defined by the method of Litchfield Wilcoxon in Swiss mice. The subchronic toxicity was evaluated by WHO and OECD's recommendation in Wistar rats with oral doses of 0.65 g/kg/day (equal to recommended human dose) and 1.95 g/kg/day (3 times as high as recommended human dose) in fourconsecutive weeks. We found that An Nguyet Khang tablet at the highest dose used for mice (35.15 g/kg) did not express acute toxicity in mice. Regarding the subchronic toxicity test, after oral administration of An Nguyet Khang tablets, hematological parameters, hepato-renal functions, and microscopic images of the liver and kidney were unchanged compared with the control group. In conclusion, An Nguyet Khang tablets did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.
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Từ khóa
An Nguyet Khang tablet, acute toxicity, subchronic toxicity, experimental animals
Tài liệu tham khảo
2. World Health Organization, Global report on traditional and complementary medicine; 2019.
3. Venkatasubbu GD, Ramasamy S, Gaddam PR, et al. Acute and subchronic toxicity analysis of surface modified paclitaxel attached hydroxyapatite and titanium dioxide nanoparticles. International Journal of Nanomedicine. 2015; 10: 137-148.
4. De Jong WH, Carraway JW, Geertsma RE. In vivo and in vitro testing for the biological safety evaluation of biomaterials and medical devices. Biocompatibility and Performance of Medical Devices. 2012; 120-158.
5. SAGANUWAN SA. Toxicity studies of drugs and chemicals in animals: an overview. Bulgarian Journal of Veterinary Medicine. 2017; 4(20): 291-318.
6. OECD, Guidelines for the testing of chemicals repeated dose oral toxicity study in rodents,. Environmental Health and Safety Monograph Series on Testing and Assesment No 407; 2008.
7. World Health Organization, Guidelines for Assessing Quality of Herbal Medicines With Reference to Contaminants and Residues. World Health Organization, Geneva; 2007.
8. Litchfield J T& Wilcoxon F A. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther. 1949; 96: 99-113.
9. World Health Organization, Working group on the safety and efficacy of herbal medicine. Report of regional office for the western pacific of the World Health Organization; 2000.
10. Lee M, Seo C, Cha S, et al. Safety assessment of So-cheong-ryong-tang: subchronic toxicity study in Crl: CD Sprague Dawley rats. Mol Med Rep. 2014; 9: 2273-2282.
11. Han JS, Lee BS, Han SR, et al. A subchronic toxicity study of Radix Dipsaci water extract by oral administration in F344 rats. Regul Toxicol Pharmacol. 2016; 81: 136-145.
12. Olson H, Betton G, Robinson D, et al. Concordance of the toxicity of pharmaceuticals in humans and in animals. Regulatory Toxicology and Pharmacology. 2000; 32(1): 56-67.
13. Chen XP, Li W, Xiao XF, et al. Phytochemical and pharmacological studies on Radix Angelica sinensis. Chin J Nat Med. 2013 Nov; 11(6): 577-87.
14. Kim D, Lee YH, Park SH, et al. Subchronic oral toxicity of evodia fruit powder in rats. J Ethnopharmacol. 2014 Feb 12; 151(3):1072-1078.