8. Antioxidant and hepatoprotective effects of hypericum sampsonii
Nội dung chính của bài viết
Tóm tắt
Hypericum sampsonii or Sampson’s St John’s Wort is a species of flowering plant in the Hypericaceae family. Our previous study revealed the diverse and bioactive chemical constituents of the plant. This study investigates the antioxidant activity and potential hepatoprotective of Hypericum sampsonii extract (BLD1 extract) in vitro and in vivo. The in vitro experiment was carried out with DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging assay to assess the antioxidant properties of BLD1 extract. In the in vivo experiment, the hepatoprotective activity of BLD1 extract was evaluated on an acetaminophen-induced hepatotoxicity mouse model. The in vitro assay demonstrated that the extract exhibited antioxidant activity in a dose-dependent manner. In the in vivo experiments, BLD1 extract at both doses (3.6 g/kg b.w day and 10.8 g/kg b.w day) reflected the decrease in serum ALT, AST and MDA level. Hepatic histology was also investigated post-mortem to assess the degree of liver injury. The overall findings of this study demonstrate the potential hepatoprotective effect of Hypericum sampsonii in acetaminophen-induced oxidative stress and liver toxicity in mice. This study has established the efficacy of Hypericum sampsonii, which should warrant further clinical trials and applications.
Chi tiết bài viết
Từ khóa
Hypericum sampsonii, BLD1 extract, hepatoprotective, antioxidant, acetaminophen, experimental animals
Tài liệu tham khảo
2. Dung Nguyen Viet, Vinh Le Ba, Thuan Nguyen Duy, et al. Bioactive compounds from the aerial parts of Hypericum sampsonii. Nat Prod Res. 2021; 35(4): 646-648. doi:10.1080/14786419.2019.1586690.
3. Cichoż-Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol WJG. 2014; 20(25): 8082-8091. doi:10.3748/wjg.v20.i25.8082.
4. Poljsak B, Šuput D, Milisav I. Achieving the Balance between ROS and Antioxidants: When to Use the Synthetic Antioxidants. Oxidative Medicine and Cellular Longevity. doi:https://doi.org/10.1155/2013/956792.
5. Okawa M, Kinjo J, Nohara T, Ono M. DPPH (1,1-Diphenyl-2- Picrylhydrazyl) Radical Scavenging Activity of Flavonoids Obtained from Some Medicinal Plants. Biol Pharm Bull. 2001; 24(10): 1202-1205. doi:10.1248/bpb.24.1202.
6. Mohamad NE, Yeap SK, Beh BK, et al. Coconut water vinegar ameliorates recovery of acetaminophen induced liver damage in mice. BMC Complement Altern Med. 2018;18:195. doi:10.1186/s12906-018- 2199-4.
7. Nguyen Thi Nga, Nguyen Trong Thong, Pham Thi Van Anh, et al. Evaluation of the antioxidant and the hepaprotective of Dysphania ambrosioides in paracetamol induce liver damage in mice. vnpharmjour. 2011; 425: 52-55.
8. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2): 351-358. doi:10.1016/0003-2697(79)90738-3.
9. Chen CL, Huang CH, Sung JM. Antioxidants in aerial parts of Hypericum sampsonii, Hypericum japonicum and Hypericum perforatum. Int J Food Sci Technol. 2009; 44(11): 2249-2255. doi:10.1111/j.1365- 2621.2009.02066.x.
10. Jaeschke H, Ramachandran A. Oxidant Stress and Lipid Peroxidation in Acetaminophen Hepatotoxicity. React Oxyg Species Apex NC. 2018; 5(15): 145-158.
11. Yoon E, Babar A, Choudhary M, Kutner M, Pyrsopoulos N. Acetaminophen-Induced Hepatotoxicity: a Comprehensive Update. J Clin Transl Hepatol. 2016; 4(2): 131-142. doi:10.14218/JCTH.2015.00052.
12. Cordiano R, Di Gioacchino M, Mangifesta R, Panzera C, Gangemi S, Minciullo PL. Malondialdehyde as a Potential Oxidative Stress Marker for Allergy-Oriented Diseases: An Update. Molecules. 2023; 28(16): 5979. doi:10.3390/molecules28165979.