9. Gastroprotective effect of Vien Khoi Tim capsules on indomethacin-induced gastric ulcers in rats

Pham Thi Van Anh, To Le Hong, Nguyen Thi Ha, Nguyen Thi Minh Thu, Pham Thanh Tung, Mai Phuong Thanh

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Tóm tắt

Available anti-ulcer drugs unveil partial effectiveness and numerous adverse reactions. Plants offer an alternative strategy in the search for new drugs in the therapy and prevention of peptic ulceration. The present study investigated the possible protective effect of the herbal formulation Vien Khoi Tim (VKT) on indomethacin-induced gastric mucosal damage in rats. VKT was tested at two doses (1.44 & 0.48 capsules/kg/d po) ten days before the indomethacin single-dose challenge (40 mg/kg po). Animals were sacrificed six hours after indomethacin administration, and gastric tissues were collected for gross observation and histopathological analyses. The results revealed that the administration of indomethacin caused evident gastric mucosal damage with morphological and histological manifestations. VKT pretreatment tended to avert the rise in lesion numbers, reduce the ulcer index, and improve the severity of bleeding streaks and epithelial sloughing in gastric mucosa on the macroscopic examination compared to the model group. It is worth noting that no ulcerative lesions were observed in the gastric tissues of rats receiving VKT upon microscopic examination. Our results indicated that Vien Khoi Tim capsules might possess a protective role against indomethacin-induced gastric ulcers. Additional research is needed to better understand the mechanism by which Vien Khoi Tim capsules exert their gastroprotective effect.

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Tài liệu tham khảo

1. Ahmed M. Peptic Ulcer Disease [Internet]. Digestive System - Recent Advances. IntechOpen; 2020. Available from: http://dx.doi.org/10.5772/intechopen.86652.
2. Bindu S, Mazumder S, Bandyopadhyay U. Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective. Biochem Pharmacol. 2020; 180:114147. doi: 10.1016/j.bcp.2020.114147.
3. Fornai M, Antonioli L, Colucci R, Tuccori M, Blandizzi C. Pathophysiology of Gastric Ulcer Development and Healing: Molecular Mechanisms and Novel Therapeutic Options [Internet]. Peptic Ulcer Disease. InTech; 2011. Available from: http://dx.doi.org/10.5772/17640.
4. Ramakrishnan K, Salinas RC. Peptic ulcer disease. Am Fam Physician. 2007; 76(7):1005-1012.
5. Musumba C, Pritchard DM, Pirmohamed M. Review article: Cellular and molecular mechanisms of NSAID-induced peptic ulcers. Aliment Pharmacol Ther. 2009; 30:517–531.
6. Huynh BV, Nguyen PNT, Nguyen NTT, Truong TQ, Phung HCV. Phytochemical analysis of Ardisia silvestris leaf extracts and their antioxidant and antibacterial activities. The Journal of Agriculture and Development. 2020; 19(4): 28-35.
7. Ly Duc Long, Tran Thi Thu Ha. Nghiên cứu đặc điểm sinh thái học và hình thái của loài khôi tía (Ardisia silvestris Pitard) tại huyện Thạch An, Cao Bằng. TNU Journal of Science and Technology. 2020; 225(11): 201 – 208.
8. Inchab K, Khonsung P, Chiranthanut N, et al. Anti-Gastric Ulcer Activity of the Water Extract from Payawanorn (Pseuderanthemum Palatiferum). Journal of Health Science and Medical Research. 2018; 36(2):89-95.
9. Kim DC, Kim SH, Choi BH, Baek NI, Kim D, Kim MJ, Kim KT. Curcuma longa extract protects against gastric ulcers by blocking H2 histamine receptors. Biol Pharm Bull. 2005; 28(12):2220-2224.
10. Guzmán-Gómez O, García-Rodríguez RV, Pérez-Gutierrez S, Rivero-Ramírez NL, García-Martínez Y, Pablo-Pérez SS, Pérez-Pastén-Borja R, Cristóbal-Luna JM, Chamorro-Cevallos G. Protective Effect of the Phycobiliproteins from Arthrospira maxima on Indomethacin-Induced Gastric Ulcer in a Rat Model. Plants (Basel). 2023;12(8):1586. doi: 10.3390/plants12081586.
11. Raish M, Shahid M, Bin Jardan YA, Ansari MA, Alkharfy KM, Ahad A, Abdelrahman IA, Ahmad A, Al-Jenoobi FI. Gastroprotective Effect of Sinapic Acid on Ethanol-Induced Gastric Ulcers in Rats: Involvement of Nrf2/HO-1 and NF-κB Signaling and Antiapoptotic Role. Front Pharmacol. 2021; 12:622815. doi: 10.3389/fphar.2021.622815.
12. Simões S, Lopes R, Campos MCD, Marruz MJ, da Cruz MEM, Corvo L. Animal models of acute gastric mucosal injury: Macroscopic and microscopic evaluation. Animal Model Exp Med. 2019; 2(2): 121-126.
13. Beiranvand M. A review of the most common in vivo models of stomach ulcers and natural and synthetic anti-ulcer compounds: A comparative systematic study. Phytomedicine Plus. 2022; 2(2): 100264. doi:10.1016/j.phyplu.2022.100264.
14. Rafatullah S, Tariq M, Al-Yahya MA, Mossa JS, Ageel AM. Evaluation of turmeric (Curcuma longa) for gastric and duodenal antiulcer activity in rats. J Ethnopharmacol. 1990; 29(1): 25-34.
15. Huang L, You L, Aziz N, et al. Antiphotoaging and Skin-Protective Activities of Ardisia silvestris Ethanol Extract in Human Keratinocytes. Plants (Basel). 2023; 12(5): 1167. doi: 10.3390/plants12051167.
16. Chayarop K, Temsiririrkkul R, Peungvicha P, et al.. Antidiabetic Effects and in vitro Antioxidant Activity of Pseuderanthemum palatiferum (Nees) Radlk. ex Lindau Leaf Aqueous Extract. Mahidol University Journal of Pharmaceutical Sciences. 2011; 38: 13-22.
17. Wang SY, Chang HN, Lin KT, Lo CP, Yang NS, Shyur LF. Antioxidant properties and phytochemical characteristics of extracts from Lactuca indica. J Agric Food Chem. 2003; 51(5):1506-1512.
18. Memarzia A, Khazdair MR, Behrouz S, et al. Experimental and clinical reports on anti-inflammatory, antioxidant, and immunomodulatory effects of Curcuma longa and curcumin, an updated and comprehensive review. Biofactors. 2021; 47(3): 311-350.
19. Zhou H, Dai C, Cui X, et al. Immunomodulatory and antioxidant effects of Glycyrrhiza uralensis polysaccharide in Lohmann Brown chickens. Front Vet Sci. 2022; 9:959449. doi: 10.3389/fvets.2022.959449.
20. Kim HJ, Seo JY, Suh HJ, Lim SS, Kim JS. Antioxidant activities of licorice-derived prenylflavonoids. Nutr Res Pract. 2012; 6(6): 491-498.