Evaluation of acute and subchronic toxicities of DEHEMA product in experimental animals

Tóm tắt

This research was conducted to evaluate the acute and sub-chronic toxicities of the DEHEMA product through oral administration using experimental animal models. The acute toxicity of DEHEMA was determined in Swiss mice using the Litchfield-Wilcoxon method. The subchronic toxicity of DEHEMA was assessed in Wistar rats according to WHO and OECD’s recommendations with oral doses of 10 mL/kg b.w./day (equivalent to the recommended human dose with conversion ratio 6) and 30 mL/kg b.w./day (3 times of the recommended human dose) for 30 consecutive days. In terms of acute toxicity, the DEHEMA product did not show acute toxicity in mice at the highest dose used (100 mL/kg b.w). In terms of subchronic toxicity, after oral administration of aqueous extract form of DEHEMA at 5.2 g/kg b.w./day and 15.6 g/kg b.w./day, the overall condition, weight, hematological parameters, liver and renal functions, and microscopic images of liver and kidney were unchanged in the treatment groups compared to those of the control group. In conclusion, DEHEMA did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.