Evaluation of acute toxicity and analgesic activities of Mekong Cissus capsules on experimental animals

Tóm tắt

Mekong Cissus capsules, a herbal preparation primarily containing Cissus quadrangularis L., were tested for both acute toxicity and pain-relieving effects in mice. The evaluation of acute oral toxicity in Swiss mice followed the procedures outlined by the WHO and the Litchfield-Wilcoxon method. Analgesic activity was assessed using three experimental models: hot plate, Von Frey, and acetic acid-induced writhing tests. No mortality or clinical sign of acute toxicity was observed, and the maximum tolerated dose was estimated at 15000 mg/kg. In the hot plate test, Mekong Cissus capsules at 288 and 864 mg/kg/day showed a tendency to prolong latency to thermal stimuli, although the changes were not statistically significant. In the Von Frey test, the higher dose (864 mg/kg/day) significantly increased both pain threshold and latency compared with baseline and control (p < 0.05), and produced a greater effect on applied force than the lower dose (p < 0.05). In the writhing test, both doses significantly reduced acetic acid-induced writhing counts from the 5th minute onward compared with the control group.