Evaluation of acute toxicity and effect of picositol in letrozole induced polycystic ovarian syndrome in animal models
Nội dung chính của bài viết
Tóm tắt
This study aims to investigate the acute toxicity and the therapeutic potential of Picositol in a letrozole-induced PCOS model in Wistar rats. An acute toxicity study of Picositol by administering a single dose (5000 mg/kg) was done in rats following OECD guideline 423. For the PCOS-induced experiment, after induction of PCOS by orally administered letrozole (1 mg/kg) for 21 days, female rats were divided into six groups for treatment up to 15 consecutive days. Blood samples were withdrawn at the end of the study for the determination of sex hormone levels, malondialdehyde (MDA), glutathione (GSH), and the relative weight of fat, uterus, and ovaries was separated. The results demonstrated that treatment with Picositol at both doses significantly reduced oxidative stress markers and increased GSH. In addition, the formulation improved endocrine disturbances by lowering testosterone and showing a tendency to increase progesterone, while no improvement in estradiol was observed. Picositol also decreased fat mass and ovarian weight and tended to increase uterine weight relative to the model group. Collectively, these findings indicate that Picositol exhibited no detectable acute toxicity and provided supportive therapeutic effects on experimental polycystic ovary syndrome in Wistar rats.
Chi tiết bài viết
Từ khóa
Picositol, acute toxicity, polycystic ovary syndrome, Wistar rats
Tài liệu tham khảo
2. Editor R. International evidence-based guideline for the assessment and management of polycystic ovary syndrome - 2023. Reproductive endocrinology. 2023; (69): 69. doi:10.18370/2309-4117.2023.69.59-79.
3. Livadas S, Anagnostis P, Bosdou JK, Bantouna D, Paparodis R. Polycystic ovary syndrome and type 2 diabetes mellitus: A state-of-the-art review. World J Diabetes. 2022; 13(1): 5-26. doi:10.4239/wjd.v13.i1.5.
4. Etrusco A, Laganà AS, Chiantera V, Buzzaccarini G, Unfer V. Myo-inositol in assisted reproductive technology from bench to bedside. Trends in Endocrinology & Metabolism. 2024; 35(1): 74-83. doi:10.1016/j.tem.2023.09.005.
5. Carrasco-Cabezas M, Assmann TS, Martínez P, et al. Folate and Vitamin B12 Levels in Chilean Women with PCOS and Their Association with Metabolic Outcomes. Nutrients. 2024; 16(12): 12. doi:10.3390/nu16121937.
6. Sparic R, Andjic M, Vergara D, et al. PCOS and vitamin D: a clinical appraisal. Arch Gynecol Obstet. 2024; 309(3): 907-915. doi:10.1007/s00404-023-07227-x.
7. Agarwal A, Sengupta P, Durairajanayagam D. Role of L-carnitine in female infertility. Reprod Biol Endocrinol. 2018; 16: 5. doi:10.1186/s12958-018-0323-4.
8. OECD. Test No. 423: Acute Oral Toxicity - Acute Toxic Class Method. OECD; 2002. doi:10.1787/9789264071001-en.
9. Reddy PS, Begum N, Mutha S, Bakshi V. Beneficial effect of Curcumin in Letrozole induced polycystic ovary syndrome. Asian Pacific Journal of Reproduction. 2016; 5(2): 116-122.
10. Cosmetic Ingredient Review, Washington, DC. Safety Assessment of Inositol as Used in Cosmetics. Final Report. In: The Expert Panel for Cosmetic Ingredient Safety. 2024.
11. Shao A, Hathcock JN. Risk assessment for the amino acids taurine, L-glutamine and L-arginine. Regul Toxicol Pharmacol. 2008; 50(3): 376-399. doi:10.1016/j.yrtph.2008.01.004.
12. Rychen G, Aquilina G, Azimonti G, et al. Safety and efficacy of l‐arginine produced by fermentation using Corynebacterium glutamicum KCCM 10741P for all animal species. EFSA J. 2018; 16(5): e05277. doi:10.2903/j.efsa.2018.5277.
13. Abbott DH, Dumesic DA, Franks S. Developmental origin of polycystic ovary syndrome - a hypothesis. J Endocrinol. 2002; 174(1): 1-5. doi:10.1677/joe.0.1740001.
14. Kafali H, Iriadam M, Ozardali I, Demir N. Letrozole-induced polycystic ovaries in the rat: a new model for cystic ovarian disease. Arch Med Res. 2004; 35(2): 103-108. doi:10.1016/j.arcmed.2003.10.005.
15. Balasubramanian A, Pachiappan S, Mohan S, Adhikesavan H, Karuppasamy I, Ramalingam K. Therapeutic exploration of polyherbal formulation against letrozole induced PCOS rats: A mechanistic approach. Heliyon. 2023; 9(5): e15488. doi:10.1016/j.heliyon.2023.e15488.
16. Sabuncu T, Vural H, Harma M, Harma M. Oxidative stress in polycystic ovary syndrome and its contribution to the risk of cardiovascular disease. Clin Biochem. 2001; 34(5): 407-413. doi:10.1016/s0009-9120(01)00245-4.
17. Liu J, Zhang D. [The role of oxidative stress in the pathogenesis of polycystic ovary syndrome]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2012; 43(2): 187-190.
18. Jadhav, Mamata et al. In vivo evaluation of Mimosa pudica Linn. in the management of polycystic ovary using rat model. International Journal of Applied Biology and P (2013): n. pag