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Published: 2022-02-22
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DOI: https://doi.org/10.52852/tcncyh.v150i2.722
Issue
Vol. 150 No. 2 (2022)
Section
Các bài báo
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Trang, T. M. ., Tâm, N. T. ., Thanh, L. V. ., Phương, P. T. M., Lan, P. T., & Doorn, H. van. (2022). Molecular mechanism of cephalosporin resistance of neisseria gonorrhoeae in Vietnam 2019 – 2020. Journal of Medical Research, 150(2), 189-201. https://doi.org/10.52852/tcncyh.v150i2.722

Molecular mechanism of cephalosporin resistance of neisseria gonorrhoeae in Vietnam 2019 – 2020

Trinh Minh Trang, Nguyen Thi Tam, Le Viet Thanh, Pham Thi Minh Phuong, Pham Thi Lan, H.Rogier van Doorn

Main Article Content

Abstract

Cephalosporin-resistant Neisseria gonorrhoeae (NG) strains have been increasing. 35 NG isolates that resisted to cephalosporin on biogram collected from 3 hospitals of dermatology and venereology in Vietnam were whole genome sequenced to determine antimicrobial resistant (AMR) genes. There were 13/35 isolates belonged to ST1901, 11/35: ST 13871. There were 16 different AMR genes. 74% of the isolates possessed mosaic penA 60.001 and 11% mosaic penA 10.001. These penA allens contained 6 key mutations responsible for ESC resistance (A311V, T483S, A501P, G545S, I312M v‡ V316T) and some novel mutations. mtrR mutants was seen in 74.2% of the isolates. All of them had Adenine deletion mutation on mtR promoter. The level of ceftriaxone resistance was relevant to FC428 strains but lower in cefixim resistance. The NG isolates (ST1901 and ST 13871) are related to cephalosporin-resistant strains detected in Japan that are currently spreading worldwide. Most of them possessed mosaic penA 60.001 and penA10.001, mtrR/mtrR promoter. These AMR genes contained typical mutations responsible for cephalosporin resistance and different novel mutations.


 

Article Details

Keywords

Cephalosporin,rr antimicrobial resistance, ARM genes, mutations, MLST...

References

1. Unemo M, Shafer WM. Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution, and future. Clin Microbiol Rev. 2014;27(3):587-613. doi:10.1128/CMR.00010-14
2. George CRR, Enriquez RP, Gatus BJ, et al. Systematic review and survey of Neisseria gonorrhoeae ceftriaxone and azithromycin susceptibility data in the Asia Pacific, 2011 to 2016. PloS One. 2019;14(4):e0213312. doi:10.1371/journal.pone.0213312
3. Ohnishi M, Golparian D, Shimuta K, et al. Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone. Antimicrob Agents Chemother. 2011;55(7):3538-3545. doi:10.1128/AAC.00325-11
4. Lee K ichi, Nakayama SI, Osawa K, et al. Clonal expansion and spread of the ceftriaxone-resistant Neisseria gonorrhoeae strain FC428, identified in Japan in 2015, and closely related isolates. J Antimicrob Chemother. 2019;74:1812-1819. doi:10.1093/jac/dkz129
5. Adamson PC, Van Le H, Le HHL, Le GM, Nguyen TV, Klausner JD. Trends in antimicrobial resistance in Neisseria gonorrhoeae in Hanoi, Vietnam, 2017–2019. BMC Infect Dis. 2020;20(1):809. doi:10.1186/s12879-020-05532-3
6. m100ed30_sample.pdf. Accessed September 10, 2021. https://clsi.org/media/3481/m100ed30_sample.pdf
7. Neisseria spp. PubMLST. Accessed August 8, 2021. https://pubmlst.org/organisms/neisseria-spp.
8. Yan J, Chen Y, Yang F, et al. High percentage of the ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone among isolates from a single hospital in Hangzhou, China. J Antimicrob Chemother. 2021;76(4):936-939. doi:10.1093/jac/dkaa526.
9. Shimuta K, Watanabe Y, Nakayama S ichi, et al. Emergence and evolution of internationally disseminated cephalosporin-resistant Neisseria gonorrhoeae clones from 1995 to 2005 in Japan. BMC Infect Dis. 2015;15:378. doi:10.1186/s12879-015-1110-x.
10. Unemo M, Nicholas RA. Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea. Future Microbiol. 2012;7(12):1401-1422. doi:10.2217/fmb.12.117.
11. Lahra MM, Martin I, Demczuk W, et al. Cooperative Recognition of Internationally Disseminated Ceftriaxone-Resistant Neisseria gonorrhoeae Strain. Emerg Infect Dis. 2018;24(4). doi:10.3201/eid2404.171873.
12. Nakayama SI, Shimuta K, Furubayashi KI, Kawahata T, Unemo M, Ohnishi M. New Ceftriaxone- and Multidrug-Resistant Neisseria gonorrhoeae Strain with a Novel Mosaic penA Gene Isolated in Japan. Antimicrob Agents Chemother. 2016;60(7):4339-4341. doi:10.1128/AAC.00504-16.
13. Ko KKK, Chio MTW, Goh SS, Tan AL, Koh TH, Abdul Rahman NB. First Case of Ceftriaxone-Resistant Multidrug-Resistant Neisseria gonorrhoeae in Singapore. Antimicrob Agents Chemother. 2019;63(5):e02624-18. doi:10.1128/AAC.02624-18.
14. Poncin T, Merimeche M, Braille A, et al. Two cases of multidrug-resistant Neisseria gonorrhoeae related to travel in south-eastern Asia, France, June 2019. Euro Surveill Bull Eur Sur Mal Transm Eur Commun Dis Bull. 2019;24(36). doi:10.2807/1560-7917.ES.2019.24.36.1900528.
15. Olsen B, Pham TL, Golparian D, Johansson E, Tran HK, Unemo M. Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from Vietnam, 2011. BMC Infect Dis. 2013;13:40. doi:10.1186/1471-2334-13-40.
16. Lan PT, Golparian D, Ringlander J, Van Hung L, Van Thuong N, Unemo M. Genomic analysis and antimicrobial resistance of Neisseria gonorrhoeae isolates from Vietnam in 2011 and 2015-16. J Antimicrob Chemother. 2020; 75(6):1432-1438. doi:10.1093/jac/dkaa040.
17. Carannante A, Vacca P, Ghisetti V, et al. Genetic Resistance Determinants for Cefixime and Molecular Analysis of Gonococci Isolated in Italy. Microb Drug Resist Larchmt N. 2017;23(2):247-252. doi:10.1089/mdr.2016.0086.