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Published: 2022-10-11
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DOI: https://doi.org/10.52852/tcncyh.v158i10.1037
Issue
Vol. 158 No. 10 (2022)
Section
Các bài báo
How to Cite
Phúc, N. V., & Chung, H. M. (2022). 1. Assay of Catapol and 5-Hydroxymethylfurfural compositions in radix rehmannie praeparatus. Journal of Medical Research, 158(10), 1-10. https://doi.org/10.52852/tcncyh.v158i10.1037

1. Assay of Catapol and 5-Hydroxymethylfurfural compositions in radix rehmannie praeparatus

Nguyen Van Phuc, Hoang Minh Chung

Main Article Content

Abstract

We conducted a qualitative and quantitative determination of catapol and 5 hydroxymethylfurfural by thin-layer chromatography and high-performance liquid chromatography for Radix Rehmanniae praeparatus processed on an industrial scale of 100kg/batch according to the Chinese Pharmacopoeia 2015 and the Korean Pharmacopoeia VIII test methods. Rhemannia glutinosa Gaertn-input material for field processing was also tested; catapol and 5 hydroxymethylfurfural were used as standards. By thin layer chromatography, the biogeochemical sample had no trace corresponding to the 5 hydroxymethylfurfural reference spot . Unstained field specimens corresponded to the catapol reference. With high-performance liquid chromatography, there was a peak corresponding to the catalpol peak, but there was no peak corresponding to 5 hydroxymethylfurfural. The content of 5 hydroxymethylfurfural in the Radix Rehmannia glutinosa praeparata surveyed samples was in the range of 0.119 - 0.134% based on dried medicinal herbs, meeting the requirements of the Korean Pharmacopoeia VIII (equal or greater than 0.1%). Based on aforementioned results, we suggest to upgrade the quantitative criteria in the Radix Rehmanniae praeparatus processed on an industrial scale.

Article Details

Keywords

Rhemannia glutinosa Gaertn, Radix Rehmanniae praeparatus, catapol, 5 hydroxymethylfurfural (5-HMF), high-performance liquid chromatography.

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