23. Creating a diabetic model with skin wounds in rats for application in wound treatment

Do Thi Quynh, Nguyen Manh Ha, Than Thi Trang Uyen, Vu Thi Thu, Dang Tran Tien, Phan Hong Minh, Ho My Dung

Main Article Content

Abstract

Chronic limb ulcer is one of many dangerous complications of diabetes mellitus. Creating a skin lesion model in rats with diabetes and hyperlipidemia opens the door for testing new treatments in clinical practice. Wistar male rats were induced to have diabetes and dyslipidemia using a high-fat diet for six weeks and low-doses Streptozocin (STZ) injections (35 mg/kg). After that, skin wounds were created by cutting the total skin layer; observation of healing process and histopathological assessment were conducted. The results showed that rats fed with a high-fat diet combined with low-dose STZ injection induced hyperlipidemia and hyperglycemia that remained above 11 mmol/L during the follow-up period. The diabetic and hyperlipidemic rats had longer wound healing time than the normal rats. Thus, this study successfully created an excisional skin wound model on Wistar rats with diabetes and hyperlipidemia which could serve in future testing phases of new wound healing treatments.

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References

1. Magliano DJ, Boyko EJ, committee IDFDAtes. IDF Diabetes Atlas. Idf diabetes atlas. International Diabetes Federation© International Diabetes Federation, 2021.; 2021.
2. Zhang P, Lu J, Jing Y, Tang S, Zhu D, Bi Y. Global epidemiology of diabetic foot ulceration: a systematic review and meta-analysis (†). Annals of medicine. Mar 2017; 49(2): 106-116. doi:10.1080/07853890.2016.1231932.
3. Rice JB, Desai U, Cummings AK, Birnbaum HG, Skornicki M, Parsons NB. Burden of diabetic foot ulcers for medicare and private insurers. Diabetes care. 2014; 37(3): 651-8. doi:10.2337/dc13-2176.
4. Cai EZ, Ang CH, Raju A, et al. Creation of consistent burn wounds: a rat model. Archives of plastic surgery. Jul 2014; 41(4): 317-24. doi:10.5999/aps.2014.41.4.317.
5. Galiano RD, Michaels Jt, Dobryansky M, Levine JP, Gurtner GC. Quantitative and reproducible murine model of excisional wound healing. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. Jul-Aug 2004; 12(4): 485-92. doi:10.1111/j.1067-1927.2004.12404.x.
6. Zhang M, Lv XY, Li J, Xu ZG, Chen L. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Experimental diabetes research. 2008; 2008:704045. doi:10.1155/2008/704045.
7. Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacological research. Oct 2005; 52(4): 313-20. doi:10.1016/j.phrs.2005.05.004.
8. Magalhães DA, Kume WT, Correia FS, et al. High-fat diet and streptozotocin in the induction of type 2 diabetes mellitus: a new proposal. Anais da Academia Brasileira de Ciencias. Mar 21 2019; 91(1): e20180314. doi:10.1590/0001-3765201920180314.
9. Furman BL. Streptozotocin-Induced Diabetic Models in Mice and Rats. Current protocols in pharmacology. Sep 1 2015; 70: 5.47.1-5.47.20. doi:10.1002/0471141755. ph0547s70.
10. Reed MJ, Meszaros K, Entes LJ, et al. A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metabolism: clinical and experimental. Nov 2000; 49(11): 1390-4. doi:10.1053/meta.2000.17721.
11. Nilsson C, Raun K, Yan FF, Larsen MO, Tang-Christensen M. Laboratory animals as surrogate models of human obesity. Acta pharmacologica Sinica. Feb 2012; 33(2): 173-81. doi:10.1038/aps.2011.203.
12. Wilkinson HN, Hardman MJ. Wound healing: cellular mechanisms and pathological outcomes. Open biology. Sep 2020; 10(9): 200223. doi:10.1098/rsob.200223.
13. Baltzis D, Eleftheriadou I, Veves A. Pathogenesis and treatment of impaired wound healing in diabetes mellitus: new insights. Advances in therapy. Aug 2014; 31(8): 817-36. doi:10.1007/s12325-014-0140-x.