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Published: 2023-10-30
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DOI: https://doi.org/10.52852/tcncyh.v170i9.1940
Issue
Vol. 170 No. 9 (2023)
Section
Các bài báo
How to Cite
Tuyết, T. T., Hiếu, V. N., & Châu, T. M. (2023). 25. Antibiotic resistance and correlation with virulence factor of helicobacter pylori at Hanoi Medical University Hospital 2019-2022. Journal of Medical Research, 170(9), 228-238. https://doi.org/10.52852/tcncyh.v170i9.1940

25. Antibiotic resistance and correlation with virulence factor of helicobacter pylori at Hanoi Medical University Hospital 2019-2022

Tran Thi Tuyet, Vu Ngoc Hieu, Tran Minh Chau

Main Article Content

Abstract

Antibiotics-resistance prevalence of H. pylori is increasing which results in difficulties in eradication of H. pylori – the leading cause to the development of gastric cancer. The purpose of this study was to evaluate the current antibiotic susceptibility of H. pylori at Hanoi Medical University Hospital from 2019 to 2023. Susceptibility testing was conducted by the Epsilometer test (E-test) method for ampicillin, clarithromycin, levofloxacin, metronidazole and tetracycline. Of the 729 biopsy samples, H. pylori was successfully cultured from 392 samples (53.7%). The isolates were tested for antibiotic susceptibility and stored at -70˚C Utra-low temperature freezer. There were 224 strains recovered by subculturing from strains that were stored at -70˚C Utra-low temperature freezer. PCR was performed on the DNA extracted from these strains for the determination of virulence factor (cagE, vacAs, vacAm, iceA1, homB). The percentage of H. pylori resistance to AMX was 67%, to CLR was 96.2%, to LVX and TE were 46% and 0.5%, respectively. Meanwhile, 100% of strains are sensitive to MTZ. The positive rates for the cagE, vacAs, vacAm, iceA1, homB genes in H. pylori were 71%; 93,3%; 69.2%; 21%; and 34.3%. A significant relationship was observed between amoxicillin resistant rate with homB(+) or cagE-homB(+) (p < 0.05).

Article Details

Keywords

Helicobacter pylori, antibiotic resistance, virulence factor

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