10. Examination of the acute and subchronic oral toxicity of “Com Kien Ty” in experimental animals
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Abstract
We evaluate the acute and subchronic toxicities of “Com kien ty” through oral administration in experimental animals. The acute toxicity was determined using the Litchfield Wilcoxon method in mice. Following WHO's recommendation, the subchronic toxicity was assessed in rabbits with oral doses of 0.9 g/kg/day (equal to the recommended human dose) and 2.7 g/kg/day (3 times as high as the recommended human dose) in 4 consecutive weeks. Results showed “Com kien ty” at the highest dose of 60.0 g/kg did not express acute toxicity in mice. Regarding the subchronic toxicity test, after oral administration of “Com kien ty”, hematological parameters, hepato-renal functions were unchanged as compared with the control group, and no gross lesions in organs were observed in all experimental animals. In conclusion, “Com kien ty” did not produce acute and subchronic toxicities in Swiss mice and New Zealand rabbits.
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Keywords
“Com kien ty”, acute toxicity, subchronic toxicity, experimental animals.
References
2. Jitareanu A., Trifan A., Vieriu M., et al. Current Trends in Toxicity Assessment of Herbal Medicines: A Narrative Review. Processes. 2022; 11(1):83.
3. Zengping Kang, Youbao Zhonga, Tiantian Wu, et al. Ginsenoside from ginseng: a promising treatment for inflammatory bowel disease. Pharmacological Reports. 2021; 73: 700-711.
4. Heraldo A.C. Rocha, Thiago V. Rocha, Fernando J.F. Nóbrega, et al. Randomized controlled trial of Panax ginseng in patients with irritable bowel syndrome. Revista Brasileira de Farmacognosia. 2018; 28(2).
5. Jiang Y, Fan L. The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancer. J Ethnopharmacol. 2021; 266:113456.
6. Ruobing Cai, Xinyi Yue, Yali Wang, et al. Chemistry and bioactivity of plants from the genus Amomum. Journal of Ethnopharmacology. 2021; 114563.
7. World Health Organization, Guidelines for Assessing Quality of Herbal Medicines With Reference to Contaminants and Residues. World Health Organization, Geneva; 2007.
8. Litchfield J T& Wilcoxon F A. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther. 1949; 96: 99-113.
9. World Health Organization, Working group on the safety and efficacy of herbal medicine. Report of regional office for the western pacific of the World Health Organization; 2000.
10. Lee M, Seo C, Cha S, et al. Safety assessment of So-cheong-ryong-tang: subchronic toxicity study in Crl: CD Sprague Dawley rabbits. Mol Med Rep. 2014; 9: 2273–2282.
11. Olson H, Betton G, Robinson D, et al. Concordance of the toxicity of pharmaceuticals in humans and in animals. Regulatory Toxicology and Pharmacology. 2000; 32(1): 56–67.
12. Sang-Jin Park, JeongHo Noh, Eun Ju Jeong, et al. Subchronic oral toxicity study of Korean red ginseng extract in Sprague-Dawley rats with a 4-week recovery period. Regulatory Toxicology and Pharmacology. 2018; 92: 83-93.
13. Fu B, Zhai X, Xi S, et al. Safety Evaluation of a New Traditional Chinese Medical Formula, Ciji-Hua’ai-Baosheng II Formula, in Adult Rodent Models. Evid Based Complement Alternat Med. 2019 Jan 14; 2019: 3659890.