20. Tác dụng hạ huyết áp và chống oxy hóa của Nano Rutin trên mô hình tăng huyết áp do N(G)-nitro-L-arginine-methyl ester (L-NAME) trên chuột cống trắng

Abstract

This study aimed to evaluate the blood pressure reducing and antioxidant effects of Nano Rutin on Wistar rats with hypertension model caused by N(G)-nitro-L-arginine-methyl ester (L-NAME). The Wistar rats were randomly allocated into 5 groups with 12 animals in each group. Group 1 was non- hypertensive rats. With the remaining 4 groups, the rats were induced to become hpertensive by orally administrating L-NAME (0.5% w/v diluted in distilled water), at 50 mg/kg b.w./day; group 2 was administered with distilled water; group 3 was administered with Losartan at 25mg/kg b.w./day; group 4 was administered with Nano Rutin at 280 mg/kg b.w./day and group 5 was administered with Nano Rutin at 560 mg/kg b.w./day. The duration to take the reagents was 4 weeks. The systolic, the diastolic pressure and the heart rate were assessed at baseline, after 1 week, 2 weeks, 3 weeks and 4 weeks of treatment. The serum levels of nitric oxide (NO) and compounds determining the anti-oxidant capacity such as Superoxide dismutase (SOD), glutathione (GSH), malondylaldehyde (MDA) were assessed at baseline and after 4 weeks of treatment. The results showed that Nano Rutin at the doses of 280 mg/kg b.w. and 560 mg/kg b.w. was effective in preventing L-NAME-induced hypertension in Wistar rats and did not alter the rats’ heart rates. In addition, Nano Rutin increased the serum NO level and the antioxidant capacity in rats administered L-NAME (increased SOD, GSH and decreased MDA).