14. So sánh hiệu quả giữa kỹ thuật microfluidic và thang nồng độ trong chuẩn bị tinh trùng ở các trường hợp làm thụ tinh trong ống nghiệm

Abstract

We conducted a comparative experimental study on 71 semen samples of couples undergoing IVF to evaluate sperm quality using 2 methods: microfluidic and density gradient centrifugation. This was a randomized controlled clinical trial study to compare embryogenesis results, and pregnancy rate between microfluidic and density gradient centrifugation techniques groups. 92 couples scheduled for IVF in the study were randomly assigned to two groups. In group 1 (intervention group), sperm was prepared by using microfluidic technique, and in group 2 (control group), sperm was prepared by using a density gradient technique. The results showed that, sperm quality obtained by both techniques is equivalent in concentration, progressive motile, recovery, and progressive sperm recovery rate. However, there was a higher vitality (97.9% vs 97.9%; p = 0.0009) and a lower DFI rate (0.64 vs 2.3; p = 0.0028) in sperm prepared by the microfluidic technique. (Based on the aforementioned result, microfluidic reduced sperm DFI is significantly better than the density gradient centrifugation method. However, there was no difference in fertilization, embryogenesis, good embryo as well as pregnancy rate between 2 groups.