Article Sidebar

PDF (Tiếng Việt)
Published: 2024-09-11
Abstract Views: 77
Views PDF (Tiếng Việt): 59
DOI: https://doi.org/10.52852/tcncyh.v180i7.2565
Issue
Vol. 180 No. 7 (2024)
Section
Các bài báo
How to Cite
Hà, V. T. ., Trung, N. V. ., Đức, N. M. ., Phượng, Đoàn T. K. ., Nhung, N. T. ., Vui, V. T. ., Minh, P. Đình ., & Huyền, N. T. . (2024). 7. Assessment of chromosomal aneuploidy in human sperm by fluorescence in situ hybridization (FISH) method. Journal of Medical Research, 180(7), 54-61. https://doi.org/10.52852/tcncyh.v180i7.2565

7. Assessment of chromosomal aneuploidy in human sperm by fluorescence in situ hybridization (FISH) method

Vu Thi Ha, Nguyen Viet Trung, Nguyen Minh Duc, Doan Thi Kim Phuong, Nguyen Thuy Nhung, Vuong Thi Vui, Pham Dinh Minh, Nguyen Thi Huyen

Main Article Content

Abstract

According to recent studies, sperm aneuploidy is a factor that can significantly affect men’s fertility, as well as the outcomes of assisted reproductive techniques. The most optimal and widely used technique for detecting sperm aneuploidy is fluorescence in situ hybridization (FISH). This study aims to assess the rate of sperm aneuploidy in males in Vietnam. We used the fluorescence in situ hybridization method to evaluate the aneuploidy of some chromosomes in 35 semen samples. The results showed that the rate of sperm aneuploidy with chromosomes 13, 18, 21 were: 0,174%, 0,161% and 0,259%, respectively. The total rate of sex chromosome aneuploidy is 0,417%. The results from 35 study samples allow for an initial description of the rate of sperm aneuploidy in Vietnamese men with potential for normal reproduction, providing reference value for future research.

Article Details

Keywords

Sperm aneuploidy, fluorescence in situ hybridization, chromosomes of sperm cell

References

1. Purity Njagi, Wim Groot, Jelena Arsenijevic, et al. Financial costs of assisted reproductive technology for patients in low- and iddle-income countries: A systematic review. Human Reproduction Open. 2023;2023(2)
2. Sunder M, Leslie SW. Semen Analysis. In: StatPearls [Internet]. 2022;
3. H Tournaye, C Krausz, R D Oates. Novel concepts in the aetiology of male reproductive impairment. The lancet Diabetes & endocrinology. Jul 2017;5(7):544-553.
4. C Esquerré-Lamare, M Walschaerts, L Chansel Debordeaux, et al. Sperm aneuploidy and DNA fragmentation in unexplained recurrent pregnancy loss: A multicenter case-control study. Basic Clin Androl. 2018;28:4.
5. Pang MG, Hoegerman SF, Cuticchia AJ, et al. Detection of aneuploidy for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 13, 17, 18, 21, X and Y by fluorescence in-situ hybridization in spermatozoa from nine patients with oligoasthenoteratozoospermia undergoing intracytoplasmic sperm injection. Hum Reprod. 1999;14(5):1266-1273.
6. L Bernardini, L Gianaroli, D Fortini, et al. Frequency of hyper-, hypohaploidy and diploidy in ejaculate, epididymal and testicular germ cells of infertile patients. Hum Reprod. Oct 2000;15(10):2165-72.
7. Marjan Pourfahraji Fakhrabadi, Seyed Mahdi Kalantar, Fatemeh Montazeri, et al. FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss. Middle East Fertility Society Journal. 2020/07/01 2020;25(1):23.
8. H G Tempest, D J Gillott, M Grigorova, et al. Sperm aneuploidy: when to stop counting? Fertility and Sterility. 2009;92(3):S141-S142.
9. C. Templado L Uroz, A Estop. New insights on the origin and relevance of aneuploidy in human spermatozoa. Molecular Human Reproduction. 2013;19(10):634-643.
10. Maj A Hultén, Suketu D Patel, Magnus Westgren, et al. On the paternal origin of trisomy 21 Down syndrome. Molecular Cytogenetics. 2010/02/23 2010;3(1):4.
11. Michaela Neusser, Nina Rogenhofer, Stephanie Dürl, et al. Increased chromosome 16 disomy rates in human spermatozoa and recurrent spontaneous abortions. Fertility and Sterility. 2015;104(5):1130-1137.e10.
12. J Jurewicz, M Radwan, W Sobala, et al. Lifestyle factors and sperm aneuploidy. Reproductive biology. Sep 2014;14(3):190-9.
13. M Radwan, J Jurewicz, B Wielgomas, et al. The association between environmental exposure to pyrethroids and sperm aneuploidy. Chemosphere. Jun 2015;128:42-8.