Androgenic effects of alcoholic extract from Cnidium monnieri (L.) Cuss fruits in experimental animals
Main Article Content
Abstract
Traditional medicine used the fruits of Cnidium monnieri (L.) Cuss to treat male hypogonadism and male infertility. This study was carried out to investigate the potential androgenic properties of the alcoholic extract of Cnidium monnieri (L.) Cuss fruits (CFE) in peripubertal castrated male rats. Rats were randomly divided into 5 groups and castrated 7 days before being administered 0.5% CMC, testosterone and CFE at 50 mg/kg/day, 150 mg/kg/day and 250 mg/kg/day, respectively, for 10 days. The relative weight of five target androgen-dependent organs (including seminal vesicles (SV), ventral prostate (VP), paired Cowper’s glands (COW), glans penis (GP) and levator ani-bulbocavernosus (LABC) muscle and blood testosterone level were evaluated. Our results showed that CFE at 150 mg/kg/day and 250 mg/kg/day increased the weights of two or more target organs and blood testosterone level. In conclusion, CFE at both doses of 150 mg/kg/day and 250 mg/kg/day has the androgenic activity in male rats.
Article Details
Keywords
Cnidium monnieri (L.) Cuss fruit, target androgen-dependent organs, testosterone, Wistar rats
References
2. Jayasena CN, Anderson RA, Llahana S, et al. Society for Endocrinology guidelines for testosterone replacement therapy in male hypogonadism. Clin Endocrinol (Oxf). 2022;96:200-219.
3. Krzastek SC, Smith RP. Non-testosterone management of male hypogonadism: an examination of the existing literature. Transl Androl Urol. 2020;9(Suppl 2):S160-S170.
4. Do Tat Loi. Vietnamese medicinal plants and herbs. Hanoi, Medical Publishing House. 2004;82.
5. James Chen, Wen-fei Chiou, Chen-Chih Chen, et al. Effect of the plant-extract osthol on the relaxation of rabbit corpus cavernosum tissue in vitro. The Journal of Urology. 2000;163:1975-1980.
6. Yuan J, Xie J, Li A, et al. Effects of Osthole on Androgen level and nitric oxide synthetase activity in castrated rats. Zhong Yao Cai. 2004;27(7):504-506.
7. Xie Jin-xian, Wang Nai-ping, Li Ping, et al. Effects of Osthol on Serum Testosterone and the Testis Androgen receptor (AR) in the reproductive system disturbance mice. Liaoning Journal of Traditional Chinese Medicine. 2007.
8. OECD 441. OECD guidelines for the testing of chemicals: Hershberger Bioassay in Rats: A Short-term Screening Assay for (Anti) Androgenic Properties. 2006.
9. OECD 115. OECD guidelines for the testing of chemicals: Hershberger Bioassay in Rats: A Short – term Screening Assay for (Anti) Androgenic Properties. 2009.
10. Kwak BK, Lee SH. Intratesticular injection of hypertonic saline: non-invasive alternative method for animal castration model. Dev Reprod. 2014;17 Suppl 4:435-40.
11. Dohle GR, Smit M, Weber RF. Androgens and male fertility. World J Urol. 2003;21(5):341-5.
12. Hiort O. The differential role of androgens in early human sex development. BMC Med. 2013;11:152.
13. Tran My Tien, Nguyen Mai Thanh Tam, Tran Cong Luan, et al. Study on the male gonadotropic effects of Morinda Officinalis How. Ho Chi Minh City Medical Research Journal. 2012;16(1):192-198.
14. Pham Thi Van Anh, Vu Viet Hang, Dau Thuy Duong, et al. Androgenic properties of “Dong Trung Ha Thao Sapa” hard capsules on experimental animals. Journal of Medical Research. 2022;154(6):153-160.
15. Pham Huu Dien, Nguyen Thi Nhan, Hoang Thi Le Thuy, et al. Main constituents from the seeds of Vietnamese Cnidium monnieri and cytotoxic activity. Nat Prod Res. 2012;26(22):2107-2111.