A study on the hypoglycemic effect of Callery speciosa extract in type 2 diabetic mice
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Abstract
The study evaluated the hypoglycemic effect of Callery speciosa extract (SNND) on Swiss mice with type 2 diabetes mellitus induced by a high-fat diet combined with streptozocin (STZ). Swiss mice were induced with type 2 diabetes mellitus by a high-fat diet continuously for 8 weeks combined with STZ intraperitoneal injection at 100 mg/kg; subsequently mice were given SNND extract at 2 doses of 3.6 g/kg/day and 14.4 g/kg/day within 2 weeks. The results showed that SNND extract at both tested doses lowered blood glucose levels and tended to reduce blood lipid levels including TC, TG, and LDL-C, accompanied by improved liver and pancreatic damage on microscopic examination. The dose of 14.4 g/kg of SNND extract exhibited a more significant hypoglycemic impact than the dose of 3.6 g/kg. The current findings demonstrated the blood glucose-lowering effect of SNND extract in a type 2 diabetic mice model in a dose-dependent manner.
Article Details
Keywords
Callery speciosa, diabetes, streptozocin, mice
References
2. Sapra A, Bhandari P. Diabetes. In: StatPearls. Treasure Island (FL): StatPearls Publishing; 2024 Jan. Available from: https://www.ncbi.nlm.nih.gov/books/NBK551501/
3. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109 Suppl 1(Suppl 1):69-75.
4. Gilani AH, Rahman AU. Trends in ethnopharmocology. J Ethnopharmacol. 2005; 100(1-2):43-49.
5. Rizvi SI, Mishra N. Traditional Indian medicines used for the management of diabetes mellitus. J Diabetes Res. 2013;2013:712092. doi: 10.1155/2013/712092.
6. Lam VQ, Anh H, Quan NV, et al. Cytotoxicity of Callerya speciosa Fractions against Myeloma and Lymphoma Cell Lines. Molecules. 2022;27(7):2322. doi: 10.3390/mole cules27072322.
7. IP VIETNAM. Bảo hộ chỉ dẫn địa lý “Núi Dành” cho sản phẩm Sâm Nam. https://www.ipvietnam.gov.vn/phat-trien-chi-dan-ia-ly/-/ass et_publisher/SGA9PgvmYtWI/content/bao-ho -chi-dan-ia-ly-nui-danh-cho-san-pham-sam-na m. Published August 25, 2021. Accessed July 04, 2024.
8. Vũ Đức Lợi, Nguyễn Thị Hiền, Trần Thị Hồng Nhung, và cs. Nghiên cứu thành phần hoá học của rễ củ cây Cát sâm (Millettia speciosa Champ.). Tạp chí Khoa học - Khoa học Y Dược. 2022;8(3):19-26.
9. Zhang M, Cui C, Lin Y, et al. Ameliorating effect on glycolipid metabolism and chemical profile of Millettia speciosa champ. extract. J Ethnopharmacol. 2021;279:114360. doi:10.10 16/j.jep.2021.114360
10. Kottaisamy CPD, Raj DS, Prasanth Kumar V, et al. Experimental animal models for diabetes and its related complications-a review. Lab Anim Res. 2021;37(1):23. doi: 10.1186/s42826-021-00101-4.
11. Lozano I, Van der Werf R, Bietiger W, et al. High-fructose and high-fat diet-induced disorders in rats: impact on diabetes risk, hepatic and vascular complications. Nutr Metab (Lond). 2016;13:15. doi:10.1186/s12986-016-0074-1
12. Softic S, Stanhope KL, Boucher J, et al. Fructose and hepatic insulin resistance. Crit Rev Clin Lab Sci. 2020;57(5):308-322.
13. Lian JH, Xiang YQ, Gou L, et al. The use of high-fat/carbohydrate diet-fed and Streptozotocin-treated mice as a suitable animal model of type 2 diabetes mellitus. Scandinavian Journal of Laboratory Animal Science. 2007;34(1):22-29.
14. Đỗ Huy Bích, Đặng Quang Chung, Bùi Xuân Chương, và cs. Cây thuốc và động vật làm thuốc ở Việt Nam, Tập 1. Nhà xuất bản Khoa học và kỹ thuật. 2006.
15. Wang MY, Ma WY, Wang QL, et al. Flavonoid-enriched extract from Millettia speciosa Champ prevents obesity by regulating thermogenesis and lipid metabolism in high-fat diet-induced obese C57BL/6 mice. Food Sci Nutr. 2021;10(2):445-459.
16. Liu L, Du X, Zhang Z, et al. Trigonelline inhibits caspase 3 to protect β cells apoptosis in streptozotocin-induced type 1 diabetic mice. Eur J Pharmacol. 2018;836:115-121.
17. Huang X, Liu G, Guo J, et al. The PI3K/AKT pathway in obesity and type 2 diabetes. Int J Biol Sci. 2018;14(11):1483-1496.
18. Tuan NN, Thi HN, My CLT, et al. Inhibition of α-Glucosidase, Acetylcholinesterase, and Nitric Oxide Production by Phytochemicals Isolated from Millettia speciosa-In Vitro and Molecular Docking Studies. Plants (Basel). 2022;11(3):388. doi: 10.3390/plants11030388.