Evaluation of the quality characteristics of collected adipose tissue-derived mesenchymal stem cell at Buu Dien Hospital
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Abstract
Adipose tissue is a rich source of stem cells for medical applications. However, the process of collecting and storing samples is associated with potential risks such as bacterial contamination, genetic alterations, or poor sample quality, which may compromise subsequent utilization. This study evaluated the quality of mesenchymal stem cells from adipose tissue samples collected at Buu Dien Hospital. The results of 20 adipose samples showed that the cell survival rate reached 99%. Microbiological testing recorded 1 sample with a positive result for bacteria, no sample tested positive for fungus and mycoplasma. The percentage of surface markers CD105+, CD73+, CD90+ are all above 97% and the average of negative marker is 2.06 ± 1.4%. Karyotyping from mesenchymal stem cells through multiple passage cultures showed nomal results in numbers and structure. The research results have an important significance for stem cell storage and application of stem cells in treatment.
Article Details
Keywords
Adipose tissue, stem cell storage, adipose tissue stem cell quality
References
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