Effect of coconut oil on the viability of fibroblasts in vitro
Main Article Content
Abstract
Lao Nha Que Coconut Oil is a product of virgin coconut oil with the intended clinical indication for the treatment of skin diseases. The study objective was to evaluate the effect of Lao Nha Que Coconut Oil on the viability of fibroblasts in vitro. From the original concentration of Coconut oil of 100%, an emulsion mixture of Coconut Oil - Tween 80 - DMEM HG was prepared at different concentrations and MTT assay was carried out on STO mouse fibroblasts. The OD absorbance at 570 nm was measured to calculate the viability of cells and the cell images were observed under the microscope. The study results showed that the concentrations of Coconut oil at IC0, IC10, and IC50 were 0.018% (v/v), 0.053% (v/v) and 0.122% (v/v), respectively.
Article Details
Keywords
Dầu dừa, quá trình lành vết thương, nguyên bào sợi, MTT, khả năng sống của tế bào
References
2. Umate, Nishigandha; Kuchewar et al. A narrative review on use of virgin coconut oil in dermatology. Journal of Indian System of Medicine. 2022; 10(2):86-89.
3. Gurtner, GC; Werner S; Barrandon Y, et al. Wound repair and regeneration. Nature. 2008, 453:314-321.
4. Ellis S; Lin EJ.; Tartar D. Immunology of Wound Healing. Curr. Dermatol. Rep. 2018, 7: 350-358.
5. Berry CE, Brenac C, Gonzalez CE et al. Natural Compounds and Biomimetic Engineering to Influence Fibroblast Behavior in Wound Healing. International Journal of Molecular Sciences. 2024; 25(6):3274.
6. Ahmad Zunairah, Sarmidi MR, et al. Evaluation of wound closure activity of cocos nucifera oil on scratched monolayer of human dermal fibroblasts. Chemical Engineering Transactions. 2017; 56:1657-1662.
7. Kamiloglu Senem, Sari Gulce, et al. Guidelines for cell viability assays. Food Frontiers. 2020; 1(3):332-349.
8. Varma Sandeep R, Sivaprakasam Thiyagarajan O, et al. In vitro anti-inflammatory and skin protective properties of Virgin coconut oil. Journal of traditional and complementary medicine. 2019; 9(1): 5-14.