Tác dụng chống oxy hoá của cao chiết hạt cà phê xanh Việt Nam trên mô hình chuột nhắt trắng gây lão suy bằng D-Galactose

Abstract

The study was conducted to evaluate the antioxidant effect of Vietnamese green coffee bean extract (VGCE) on D-galactose – induced aging mice model. The male white mice of Swiss strain were divided into 6 groups: group 1 (control, n = 12, subcutaneous injection of 9‰ NaCl 0.1 ml/10g + oral administration of 9‰ NaCl); group 2 (disease, n = 13, subcutaneous injection of 100 mg/kg D-galactose + oral administration of 9‰ NaCl); group 3 (positive control, n = 10, subcutaneous injection of 100 mg/kg D-galactose + oral administration of 50 mg/kg Vitamin E); group 4 (VGCE200, n = 12, subcutaneous injection of 100 mg/kg D-galactose + oral administration of 200 mg/kg VGCE); group 5 (VGCE300, n = 10, subcutaneous injection of 100 mg/kg D-galactose + oral administration of 300 mg/kg VGCE); group 6 (VGCE400, n = 13, subcutaneous injection of 100 mg/kg D-galactose + oral administration of 400 mg/kg VGCE). After treatment blood was collected to quantify plasma MDA, GPx, and SOD by ELISA technique. SOD concentration of VGCE400 group increased statistically significantly compared to the disease group. In conclusion, at 400 mg/kg, Vietnamese green coffee bean extract showed antioxidant effects by increasing SOD concentration in senescent white mice.