So sánh tương đồng kết quả xét nghiệm một số chỉ số hóa sinh giữa hai máy hóa sinh tự động Olympus AU640 và DxC 700AU

Abstract

Laboratories with multiple analyzers and testing procedures need to compare test results to evaluate the equivalence of assays within the same system. This study was conducted to assess the comparability of routine biochemical test results of urea, creatinine, AST, ALT, cholesterol, triglycerides, and glucose-between the Olympus AU640 and the DxC 700AU Beckman Coulter analyzers at the Agricultural General Hospital. The study was performed using leftover plasma samples after routine testing, following an experimental laboratory design based on CLSI guideline EP09. Each sample was analyzed once on each analyzer, and the Method Validator software by Philip Marquez was used to assess the comparability of 40 patient samples between the two analyzers via Bland-Altman difference plots. Further comparisons were conducted using both Bland-Altman plots and Passing–Bablok regression. The results showed that all seven test parameters (triglycerides, cholesterol, creatinine, urea, glucose, AST, and ALT) had strong correlations and mean biases within the allowable error limits defined by CLIA when compared between the Olympus AU640 and DxC 700AU analyzers. We conclude that the laboratory can alternately use the two analyzers in routine practice or consider establishing an internal correction factor based on the derived regression equations. However, it is recommended to implement an internal protocol to manage systematic bias between the two systems.