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Published: 2025-07-28
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DOI: https://doi.org/10.52852/tcncyh.v192i7.3636
Issue
Vol. 192 No. 7 (2025)
Section
Các bài báo
How to Cite
Việt, T. M., Lan, N. T. N., Mến, Đỗ T., Trung, N. T., Linh, N. N., Tùng, V. T., & Tuyên, Đỗ T. (2025). 47. Extraction and preliminary purification of lovastatin from the fermentation broth of monascus purpureus strain. Journal of Medical Research, 192(7), 448-457. https://doi.org/10.52852/tcncyh.v192i7.3636

47. Extraction and preliminary purification of lovastatin from the fermentation broth of monascus purpureus strain

Trinh Minh Viet, Nguyen Thi Ngoc Lan, Do Tuan Men, Nguyen Thi Trung, Nguyen Nhat Linh, Vu Thanh Tung, Do Thi Tuyen

Main Article Content

Abstract

As a HMG-CoA reductase inhibitor, lovastation has an important role in lowering blood cholesterol levels. The purpose of this study was to optimize the cultivation and extraction conditions for lovastatin production from Monascus purpureus to improve the yield through a biological approach, as an alternative to a less environmentally friendly chemical synthesis. The results showed that malt extract broth (MEB) was the most effective medium, yielding the highest lovastatin production (82.9 µg/mL), which is 7.8 times higher than the potato dextrose broth (PDB). Lovastatin was extracted using ethyl acetate at pH 3, identified by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), and partially purified using silica gel column chromatography with a dichloromethane/ethyl acetate (7/3, v/v) solvent system. The resulting fractions contained 42.08 - 64.16% lovastatin. These findings provide a foundation for further studies on the purification and medical application of lovastatin.

Article Details

Keywords

Monascus purpureus, lovastatin, purification, chromatography

References

1. Fiorentino R, Chiarelli F. Statins in children, an update. International Journal of Molecular Sciences. 2023; 24(2): 1366.
2. Tang X-L, Yu J-W, Geng Y-H, Wang J-R, Zheng R-C, Zheng Y-G. From Discovery to Mass Production: A Perspective on Bio-Manufacturing Exemplified by the Development of Statins.Engineering. 2023/05/01/2023; 24: 138-150. doi:https://doi.org/10.1016/j.eng.2022.04.030.
3. Immanuel S, Anusha P. Production of lovastatin by soil microfungi Rhizopus oryzae. Acta Scientific Med Sci. 2019; 3(1): 70-74.
4. Panda BP, Javed S, Ali M. Optimization of fermentation parameters for higher lovastatin production in red mold rice through co-culture of Monascus purpureus and Monascus ruber. Food and Bioprocess Technology. 2010; 3: 373-378.
5. Dhar R, Nigam K. Studies on process parameters for production of lovastatin. Asian Journal of Biomedical and Pharmaceutical Sciences. 2015; 5(51): 25.
6. Zhang S, Zeng X, Lin Q, Liu J. Analysis of secondary metabolite gene clusters and chitin biosynthesis pathways of Monascus purpureus with high production of pigment and citrinin based on whole-genome sequencing. Plos one. 2022; 17(6): e0263905.
7. Manzoni M, Rollini M, Bergomi S, Cavazzoni V. Production and purification of statins from Aspergillus terreus strains. Biotechnology Techniques. 1998/07/01 1998; 12(7): 529-532. doi:10.1023/A:1008851430560.
8. Lisec B, Radež I, Žilnik LFJS, technology p. Solvent extraction of lovastatin from a fermentation broth. 2012; 96: 187-193.
9. Xiong Z, Cao X, Wen Q, et al. An overview of the bioactivity of monacolin K/lovastatin. 2019;131:110585.
10. Immanuel S, Anusha PJASMS. Production of lovastatin by soil microfungi Rhizopus oryzae. 2019; 3(1): 70-74.
11. Upendra R, Pratima K, Amiri Z, Shwetha L, Ausim MJJMBT. Screening and molecular characterization of natural fungal isolates producing lovastatin. 2013; 5(2):25-30.