15. Selected factors associated with sperm dna fragmentation in male undergoing in vitro fertilization
Main Article Content
Abstract
A cross-sectional study was conducted on 120 infertile couples undergoing in vitro fertilization to investigate the association between semen parameters, selected clinical factors, and the sperm DNA fragmentation index (DFI). The results showed that DFI was significantly and negatively correlated with progressive motility and sperm viability (p < 0.05). Age demonstrated a positive correlation with DFI (p < 0.05), whereas other factors such as BMI, duration of infertility, history of smoking, and antioxidant use were not significantly associated with DFI. These findings indicate that DFI is related to sperm quality and may be influenced by certain risk factors to varying degrees. The results suggest the complementary role of DFI in the diagnosis of male infertility and emphasize the need for larger-scale studies to further elucidate these associations.
Article Details
Keywords
Sperm DNA fragmentation, DFI, semen analysis, male infertility
References
2. Kim NI, Chamchan C, Tangchonlatip K. Prevalence and Social Risk Factors of Infertility in Vietnam. Illn Crisis Loss. 2022; 30(4): 756-769.
3. Agarwal A, Allamaneni SSR. Sperm DNA damage assessment: a test whose time has come. Fertil Steril. 2005; 84(4): 850-853.
4. Muriel L, Garrido N, Fernández JL, et al. Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracytoplasmic sperm injection. Fertil Steril. 2006; 85(2): 371-383.
5. Henkel R, Hajimohammad M, Stalf T, et al. Influence of deoxyribonucleic acid damage on fertilization and pregnancy. Fertil Steril. 2004; 81(4): 965-972.
6. Cho CL, Agarwal A. Role of sperm DNA fragmentation in male factor infertility: A systematic review. Arab J Urol. 2018; 16(1): 21-34.
7. Sivanarayana T, Ravi Krishna C, Jaya Prakash G, et al. Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection. Reprod Med Biol. 2014; 13(2): 87-94.
8. WHO. WHO laboratory manual for the examination and processing of human semen, 5th ed. Published online 2010.
9. Borges E, Zanetti BF, Setti AS, Braga DPDAF, Provenza RR, Iaconelli A. Sperm DNA fragmentation is correlated with poor embryo development, lower implantation rate, and higher miscarriage rate in reproductive cycles of non-male factor infertility. Fertil Steril. 2019; 112(3): 483-490.
10. Vinnakota C, Cree L, Peek J, Morbeck DE. Incidence of high sperm DNA fragmentation in a targeted population of subfertile men. Syst Biol Reprod Med. 2019; 65(6): 451-457.
11. Nguyen Thi Hiep T, Nguyen Van T, Nguyen Thi Thai T, Dang Thi Hong N, Dang Cong T, Le Minh T. Sperm dna fragmentation and the relationship with semen parameters. J Med Pharm. Published online October 29, 2021: 112-118.
12. Evenson DP, Djira G, Kasperson K, Christianson J. Relationships between the age of 25,445 men attending infertility clinics and sperm chromatin structure assay (SCSA®) defined sperm DNA and chromatin integrity. Fertil Steril. 2020; 114(2): 311-320.
13. Moskovtsev SI, Willis J, Mullen JBM. Age-related decline in sperm deoxyribonucleic acid integrity in patients evaluated for male infertility. Fertil Steril. 2006; 85(2): 496-499.
14. Liu K, Mao X, Pan F, Chen Y, An R. Correlation analysis of sperm DNA fragmentation index with semen parameters and the effect of sperm DFI on outcomes of ART. Sci Rep. 2023; 13(1): 2717.
15. Osadchuk L, Kleshchev M, Osadchuk A. Effects of cigarette smoking on semen quality, reproductive hormone levels, metabolic profile, zinc and sperm DNA fragmentation in men: results from a population-based study. Front Endocrinol. 2023; 14: 1255304.
16. Romano M, Cirillo F, Spadaro D, et al. High sperm DNA fragmentation: do we have robust evidence to support antioxidants and testicular sperm extraction to improve fertility outcomes? a narrative review. Front Endocrinol. 2023;14: 1150951.
17. Szabó A, Váncsa S, Hegyi P, et al. Lifestyle-, environmental-, and additional health factors associated with an increased sperm DNA fragmentation: a systematic review and meta-analysis. Reprod Biol Endocrinol. 2023; 21(1): 5.