Article Sidebar

PDF (Tiếng Việt)
Published: 2025-12-30
Abstract Views: 0
Views PDF (Tiếng Việt): 0
DOI: https://doi.org/10.52852/tcncyh.v197i12.4437
Issue
Vol. 197 No. 12 (2025)
Section
Các bài báo
How to Cite
Lam, N. T. T., Lan, B. N., Hà, Đặng T., Anh, N. H., Dương, Đặng Ánh, Ngọc, N. B., Phương, H. T., Minh, L. Đức, Thoa, T. T., Hạnh, T. T. T., Thạch, N. C., & Bình, N. T. (2025). Outcomes of autologous peripheral blood hematopoietic stem cell collection in pediatric patients with neuroblastoma. Journal of Medical Research, 197(12), 316-325. https://doi.org/10.52852/tcncyh.v197i12.4437

Outcomes of autologous peripheral blood hematopoietic stem cell collection in pediatric patients with neuroblastoma

Nguyen Thi Thanh Lam, Bui Ngoc Lan, Dang Thi Ha, Nguyen Hoai Anh, Dang Anh Duong, Nguyen Bao Ngoc, Ha Thi Phuong, Le Duc Minh, Ta Thi Thoa, Tran Thi Thuy Hanh, Nguyen Co Thach, Nguyen Thanh Binh

Main Article Content

Abstract

Neuroblastoma is the most common extracranial solid tumor in children. The high-risk group represents one of the most challenging pediatric malignancies for treatment. In the treatment protocol, autologous hematopoietic stem cell transplantation following high-dose chemotherapy helps restore hematopoiesis and bone marrow function in this patient group. This study aimed to describe the outcomes of peripheral blood stem cell collection in pediatric patients with high-risk neuroblastoma undergoing autologous hematopoietic stem cell transplantation. A cross-sectional descriptive study was conducted on 57 patients diagnosed with high-risk neuroblastoma who underwent autologous peripheral blood stem cell collection at the Vietnam National Children’s Hospital. Before apheresis, the median peripheral white blood cell count was 20.04×109/L (IQR 13.33 - 26.87), and the median peripheral CD34+ cell count was 32 cells/µL (IQR 18.72 - 48.53) with a statistically significant positive correlation with monocyte counts. The collected peripheral blood stem cell collection products had a median total nucleated cell count of 29.35×109 cells (IQR 19.39 - 38.41) and median number of CD34+ hematopoietic stem cells count of 84.25×106 cells (IQR 54.54 - 136.99), median CD34+ stem cell dose of 6.29×106 cells/kg (IQR 4.43 - 9.04). The collected peripheral blood stem cell collection grafts met target requirements for total cell count, cell density, and CD34+ dose, providing adequate quality for high-dose chemotherapy support in pediatric patients with high-risk neuroblastoma.

Article Details

Keywords

High-risk neuroblastoma, autologous hematopoietic stem cell transplantation, Vietnam National Children’s Hospital

References

1. Yan J, Jie L, Jiaxing Y, et al. Analysis of the efficacy of autologous peripheral blood stem cell transplantation in high-risk neuroblastoma. Int J Med Sci. 2022; 19(11): 1715-1723. doi:10.7150/ijms.76305.
2. Park JR, Kreissman SG, London WB, et al. Effect of tandem autologous stem cell transplant vs single transplant on event-free survival in patients with high-risk neuroblastoma: a randomized clinical trial. JAMA. 2019; 322(8): 746-755. doi:10.1001/jama.2019.11642.
3. Karow A, Wilhelm A, Ammann RA, Baerlocher GM, Pabst T, Mansouri Taleghani B, et al. Peripheral blood progenitor cell collection in pediatric patients optimized by high pre-apheresis count of circulating CD34+ cells and high blood flow. Bone Marrow Transplant. 2019; 54(6): 885-893. doi:10.1038/s41409-018-0382-4.
4. Grupp SA, Cohn SL, Wall D, Reynolds CP; Hematopoietic Stem Cell Transplant Discipline and the Neuroblastoma Disease Committee, Children’s Oncology Group. Collection, storage, and infusion of stem cells in children with high-risk neuroblastoma: saving for a rainy day. Pediatr Blood Cancer. 2006; 46(7): 719-722. doi:10.1002/pbc.20769.
5. Lee CY, Yu TY, Lin FL, Hung GY, Hou MH, Ho CY, Liu CY, Chiou TJ, Yen HJ. Peripheral blood stem cell harvesting in young children weighing less than 15 kg: a single-institute experience in Taiwan. Cytotherapy. 2024; 26(10): 1201-1209. doi:10.1016/j.jcyt.2024.05.008.
6. Melve GK, Ersvaer E, Eide GE, Kristoffersen EK, Bruserud Ø. Peripheral blood stem cell mobilization in healthy donors by granulocyte colony-stimulating factor causes preferential mobilization of lymphocyte subsets. Front Immunol. 2018; 9: 845. doi:10.3389/fimmu.2018.00845.
7. Kilpeläinen L, Nikkilä A, Lohi O. Pre-harvest CD34+ cell counts predicted peripheral blood stem cell collection yields. Acta Paediatr. 2023; 112(10): 1999-2005. doi:10.1111/apa.16879.
8. Park SD, Saunders AS, Reidy MA, Bender DE, Clifton S, Morris KT. A review of granulocyte colony-stimulating factor receptor signaling and regulation with implications for cancer. Front Oncol. 2022; 12: 932608. doi:10.3389/fonc.2022.932608.
9. Bendall LJ, Bradstock KF. G-CSF: from granulopoietic stimulant to bone marrow stem cell mobilizing agent. Cytokine Growth Factor Rev. 2014; 25(4): 355-367. doi:10.1016/j.cytogfr.2014.07.011.
10. Martino M, Gori M, Pitino A, et al. Basal CD34+ cell count predicts peripheral blood stem cell mobilization in healthy donors after administration of granulocyte colony-stimulating factor: a longitudinal, prospective, observational, single-center, cohort study. Biol Blood Marrow Transplant. 2017; 23(7): 1215-1220. doi:10.1016/j.bbmt.2017.03.024.
11. Okano A, Ashihara E, Shimazaki C, et al. Predictive parameters for granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization. J Clin Apher. 2008; 23(6): 179-184. doi:10.1002/jca.20179.
12. Pelus LM, Bian H, King AG, Fukuda S. Neutrophil-derived MMP-9 mediates synergistic mobilization of hematopoietic stem and progenitor cells by the combination of G-CSF and the chemokines GROβ/CXCL2 and GROβT/CXCL2Δ4. Blood. 2004; 103(1): 110-119. doi:10.1182/blood-2003-04-1115. PMID:12958067.
13. Stroncek DF, Clay ME, Herr G, et al. The kinetics of G-CSF mobilization of CD34+ cells in healthy people. Transfus Med. 2003; 7(1): 19-24. doi:10.1046/j.1365-3148.1997.d01-75.x.
14. Sharma N, Sawant RB, Sen S. Optimizing pediatric peripheral blood stem cell collection. Transfus Apher Sci. 2021; 60(1): 102966.
15. Güngörer V, Yılmaz Keskin E, Fışgın T. Retrospective evaluation of children after stem cell transplantation: single center experience. J Contemp Med. 2019; 9(4): 359-364. doi:10.16899/jcm.605404.