Đánh giá mức độ biến thiên giữa các phương pháp một số xét nghiệm ngoại kiểm sinh hóa năm 2024

Abstract

Many previous studies have evaluated the accuracy of each testing method, however, surveys from multiple external quality assessment data sets to reflect the variability between methods under real-world conditions have been lacking. This study aims to assess the variability of methods for quantifying Albumin, ALT, Glucose, and HDL-Cholesterol based on CV% data from 12 batches of external quality assessment in 2024. The results show that the BCG method for Albumin quantification has a more stable CV% than BCP (IQR 3.81 – 4.75%). For ALT, Tris buffer without P5P has a lower and more stable median CV% than Tris buffer with P5P. For glucose, hexokinase had the lowest CV% (2.59%) and was more stable than glucose oxidase and glucose dehydrogenase. For HDL-cholesterol, Direct HDL-immunoseparation showed the lowest CV% (6.63%) and was more stable than the Clearance method and Polyethylene Glycol Modified Enzyme Method. The study results provide evidence of the variability between measurement methods, supporting laboratories in selecting a method with higher stability to improve test quality.