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Published: 2026-03-31
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DOI: https://doi.org/10.52852/tcncyh.v200i3.4783
Issue
Vol. 200 No. 3 (2026)
Section
Các bài báo
How to Cite
Hằng, Đinh T. T., Anh, P. T. V., Xuân, H. T. T., Tranh, Đàm Đình, & Dương, Đậu T. (2026). Effects of Domuvar (Bacillus subtilis THC1I) on intestinal motility in experimental animals. Journal of Medical Research, 200(3), 408-415. https://doi.org/10.52852/tcncyh.v200i3.4783

Effects of Domuvar (Bacillus subtilis THC1I) on intestinal motility in experimental animals

Dinh Thi Thu Hang, Pham Thi Van Anh, Ho Thi Thanh Xuan, Dam Dinh Tranh, Dau Thuy Duong

Main Article Content

Abstract

Domuvar is a probiotic formulation with Bacillus subtilis THC1I as its primary component. The study was conducted to evaluate its effects on intestinal motility using an isolated rabbit intestine model and a charcoal gastrointestinal transit model in mice. Isolated intestinal segments were divided into three groups with Enterogermina, Domuvar (Bacillus subtilis THC1I) at two concentrations of 4 × 107 CFU/mL Tyrode and 8 × 107 CFU/mL Tyrode, and the frequency as well as the amplitude of intestinal contractions were assessed. In the murine model, gastrointestinal transit of charcoal was compared among groups administered distilled water, Enterogermina, and Domuvar at 0.82 × 109 CFU/kg or 1.64 × 109 CFU/kg. The results demonstrated that Domuvar reduced intestinal motility in both experimental models, and the effect was dose-dependent. These findings suggest that Domuvar (Bacillus subtilis THC1I) may be a promising therapeutic product for the management of clinical conditions associated with increased intestinal motility, such as diarrhea.

Article Details

Keywords

Bacillus subtilis THC1I, intestinal motility, isolated intestine model, charcoal gastrointestinal transit

References

1. Dimidi E, Christodoulides S, Scott SM, et al. Mechanisms of Action of Probiotics and the Gastrointestinal Microbiota on Gut Motility and Constipation. Adv Nutr. 2017; 8(3): 484-494. doi:10.3945/an.116.014407.
2. Hotel A. Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria - Joint FAO/WHO Expert Consultation. 2001; 2014.
3. Hill C, Guarner F, Reid G, et al. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014; 11(8): 506-514. doi:10.1038/nrgastro.2014.66.
4. Parker EA, Roy T, D’Adamo CR, et al. Probiotics and Gastrointestinal Conditions: An Overview of Evidence from the Cochrane Collaboration. Nutr Burbank Los Angel Cty Calif. 2018; 45: 125-134.e11. doi:10.1016/j.nut.2017.06.024.
5. Su GL, Ko CW, Bercik P, et al. AGA Clinical Practice Guidelines on the Role of Probiotics in the Management of Gastrointestinal Disorders. Gastroenterology. 2020; 159(2): 697-705. doi:10.1053/j.gastro.2020.05.059.
6. Spears JL, Kramer R, Nikiforov AI, et al. Safety Assessment of Bacillus subtilis MB40 for Use in Foods and Dietary Supplements. Nutrients. 2021; 13(3): 733. doi:10.3390/nu13030733.
7. Errington J, van der Aart LT. Microbe Profile: Bacillus subtilis: model organism for cellular development, and industrial workhorse. Microbiology. 2020; 166(5): 425-427. doi:10.1099/mic.0.000922.
8. Franz J. Hock. Drug Discovery and Evaluation: Pharmacological Assays. Springer Cham; 2015.
9. Waclawiková B, Codutti A, Alim K, et al. Gut microbiota-motility interregulation: insights from in vivo, ex vivo and in silico studies. Gut Microbes. 14(1): 1997296. doi:10.1080/19490976.2021.1997296.
10. Jespersen B, Tykocki NR, Watts SW, et al. Measurement of smooth muscle function in the isolated tissue bath-applications to pharmacology research. J Vis Exp JoVE. 2015; (95): 52324. doi:10.3791/52324.
11. Earnest E, Emudainohwo T, Igboeme S, et al. Preclinical Screening Techniques for Antidiarrheal Drugs: A comprehensive review. Am J Physiol Biochem Pharmacol. 2018; 7: 61. doi:10.5455/ajpbp.20180329014330.
12. Padmanabhan P, Grosse J, Asad ABMA, et al. Gastrointestinal transit measurements in mice with 99mTc-DTPA-labeled activated charcoal using NanoSPECT-CT. EJNMMI Res. 2013; 3: 60. doi:10.1186/2191-219X-3-60.
13. Acosta-Rodríguez-Bueno CP, Abreu Y Abreu AT, Guarner F, et al. Bacillus clausii for Gastrointestinal Disorders: A Narrative Literature Review. Adv Ther. 2022; 39(11): 4854-4874. doi:10.1007/s12325-022-02285-0.
14. Gong P, Tang X. The impact of probiotic supplementation on gastric motility and nutrient absorption in elderly patients with Gastrointestinal disorders. BMC Gastroenterol. 2025; 25: 192. doi:10.1186/s12876-025-03740-2.
15. Bai X, Ihara E, Tanaka Y, et al. The interplay of gut microbiota and intestinal motility in gastrointestinal function. J Smooth Muscle Res Nihon Heikatsukin Gakkai Kikanshi. 2025; 61: 51-58. doi:10.1540/jsmr.61.51.