Evaluation of the effects of myo-inositol and its combinations in experimental infertility model in female mice
Main Article Content
Abstract
Infertility is an increasingly prevalent health issue that significantly affects social well-being. This study aimed to evaluate the effects of myo-inositol combined with antioxidants (S-FEMME) on follicular development and reproductive performance in an ethinyl estradiol–induced anovulatory infertility model. Female Swiss mice were divided into groups: control, model, clomiphene citrate, and S-FEMME-treated, each receiving 0.32 g/kg or 0.64 g/kg. S-FEMME treatment significantly improved estrous cycle regularity, serum estrogen levels, and fertility-related parameters compared with the model group. Histopathological examination of the ovaries revealed an increased number of developing follicles and the presence of corpora lutea in the treated groups. In addition, the high-dose S-FEMME group showed greater improvement in reproductive outcomes compared with the model group. These findings suggest that S-FEMME promotes follicular development and enhances fertility, indicating its potential as a supportive therapeutic agent for reproductive health.
Article Details
Keywords
Myo-inositol, repeated dose toxicity, infertility, Swiss mice
References
2. Cox CM, Thoma ME, Tchangalova N, et al. Infertility prevalence and the methods of estimation from 1990 to 2021: a systematic review and meta-analysis. Hum Reprod Open. 2022;2022(4):hoac051. doi:10.1093/hropen/hoac051
3. Sharma M, Balasundaram P. Ovulation Induction Techniques. In: StatPearls [Internet]. StatPearls Publishing; 2023.
4. Diamond MP, Legro RS, Coutifaris C, et al. Letrozole, Gonadotropin, or Clomiphene for Unexplained Infertility. N Engl J Med. 2015;373(13):1230-1240. doi:10.1056/NEJMoa1414827
5. Placidi M, Casoli G, Tatone C, et al. Myo-Inositol and Its Derivatives: Their Roles in the Challenges of Infertility. Biology (Basel). 2024;13(11):936. doi:10.3390/biology13110936
6. Murto T, Skoog Svanberg A, Yngve A, et al. Folic acid supplementation and IVF pregnancy outcome in women with unexplained infertility. Reprod Biomed Online. 2014;28(6):766-772. doi:10.1016/j.rbmo.2014.01.017
7. Aubead NM. Assessment of the Role of vitamin E in the improvement of female fertility. Journal of Obstetrics, Gynecology and Cancer Research. Published online August 26, 2025:e728645.
8. Zaha I, Muresan M, Tulcan C, et al. The Role of Oxidative Stress in Infertility. J Pers Med. 2023;13(8):1264. doi:10.3390/jpm13081264
9. Amudha M, Rani S. Fertility inducing activity of Cordia retusa in Female Albino rats. Asian Journal of Pharmaceutical Research. 2020;10(3):171-176. doi:10.5958/2231-5691. 2020.00030.1
10. Coelingh Bennink HJT, Skouby S, Bouchard P, et al. Ovulation inhibition by estetrol in an in vivo model. Contraception. 2008;77(3):186-190. doi:10.1016/j.contracep tion.2007.11.014
11. Quignon C. Collection and analysis of vaginal smears to assess reproductive stage in mice. Curr Protoc. 2023;3(9):e887. doi:10.1002/cpz1.887
12. Laganà AS, Garzon S, Casarin J, et al. Inositol in Polycystic Ovary Syndrome: Restoring Fertility through a Pathophysiology-Based Approach. Trends Endocrinol Metab. 2018;29(11):768-780. doi:10.1016/j.tem.2018.09.001
13. Agarwal A, Sengupta P, Durairajanayagam D. Role of L-carnitine in female infertility. Reprod Biol Endocrinol. 2018;16:5. doi:10.1186/s12958-018-0323-4
14. Tsuji A, Nakamura T, Shibata K. Biotin-deficient diet induces chromosome misalignment and spindle defects in mouse oocytes. Biosci Biotechnol Biochem. 2015;79(2):292-299. doi:10.1080/09168451.2014.968090
15. Barboriak JJ, Krehl WA, Cowgill GR, et al. Effect of Partial Pantothenic Acid Deficiency on Reproductive Performance of the Rat. The Journal of Nutrition. 1957;63(4):591-599. doi:10.1093/jn/63.4.591