Ảnh hưởng của gemcitabine lên xâm lấn và biểu hiện E-Cadherin của tế bào ung thư đường mật trong gan

Abstract

This study was conducted to investigate the effect of Gemcitabine on the invasiveness and expression of E-Cadherin in gemcitabine-resistant intrahepatic cholangiocarcinoma (iCCC) cells. Gemcitabine-resistant iCCC cells (SZ1-G28) were established and maintained in the laboratory. Invasive capacity was compared between iCCC resistant and non-resistant cells with and without gemcitabine using invasion assays. E-Cadherin protein expression in both gemcitabine-resistant and non-resistant cells was evaluated in vitro by Western blot. Gemcitabine-resistant SZ1-G28 cells exhibited significantly increased invasiveness compared to non-resistant cells. Treatment with gemcitabine 20 µg/mL promoted invasion in the parental cell line (SZ1-P). Furthermore, E-Cadherin expression was reduced in SZ1-G28 cells relative to SZ1-P cells. Gemcitabine resistance and gemcitabine treatment enhance the aggressiveness and plasticity of iCCC cells, leading to increased cellular invasiveness. Concurrently, E-Cadherin expression is downregulated in gemcitabine-resistant cells compared to the parental non-resistant cells. These findings suggest that both resistance and treatment with gemcitabine promote the invasiveness of iCCC cells, associated with decreased E-Cadherin expression.