Wound healing effect of medicell plus premium on an experimental skin injury model
Main Article Content
Abstract
Medicell Plus Premium, manufactured by Picostech Co., Lt, is a topical dermatological formulation containing 11% bone marrow–derived cell culture extract, Leontopodium alpinum cell extract, peptides, and hydrolyzed collagen. This study evaluated the wound-healing and systemic effects of Medicell Plus Premium in Swiss albino mice with experimentally induced skin injuries. Dorsal skin wounds were induced, and the animals were treated topically with Contractubex gel (0.1 g per application, twice daily) or Medicell Plus Premium (0.15 mL per application, once or twice daily) for 14 consecutive days. The results indicated that treatment with Medicell Plus Premium accelerated wound healing, as evidenced by reduced wound area, increased hydroxyproline content in the skin, and improved histopathological features at the wound site. Additionally, no significant differences were observed in general condition, haematological parameters, liver and kidney function, or the histological structure of the liver and kidneys between the Medicell Plus Premium-treated groups and the normal control group after 14 days of application. These findings indicate that Medicell Plus Premium promotes wound healing without inducing systemic toxicity when applied topically in Swiss mice with skin injuries.
Article Details
Keywords
wound healing, Medicell Plus Premium, Swiss mice
References
2. Peña OA, Martin P. Cellular and molecular mechanisms of skin wound healing. Nature Reviews Molecular Cell Biology. 2024;25(8):599-616.
3. He C, Dai M, Zhou X, et al. Comparison of two cell-free therapeutics derived from adipose tissue: small extracellular vesicles versus conditioned medium. Stem Cell Research & Therapy. 2022;13(1):86.
4. Gupta A, Cady C, Fauser AM, et al. Cell-free Stem cell-derived extract formulation for regenerative medicine applications. International Journal of Molecular Sciences. 2020;21(24).
5. Gragnani A, Tonarelli E, Chomiski V, et al. Fibroblast growth factor in the treatment of burns: A systematic review. Burns. 2022;48(1):104-10.
6. Ching YH, Sutton TL, Pierpont YN, et al. The use of growth factors and other humoral agents to accelerate and enhance burn wound healing. Eplasty. 2011;11:e41.
7. Cho WK, Kim HI, Kim SY, et al. Anti-aging effects of Leontopodium alpinum (Edelweiss) callus culture extract through transcriptome profiling. Genes (Basel). 2020;11(2).
8. Hajiaghaalipour F, Kanthimathi MS, Abdulla MA, et al. The effect of Camellia sinensis on wound healing potential in an animal model. Evidence-Based Complementary and Alternative Medicine. 2013;2013:386734.
9. Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nature Methods. 2012;9(7):671-5.
10. Schindelin J, Rueden CT, Hiner MC, et al. The ImageJ ecosystem: An open platform for biomedical image analysis. Molecular Reproduction and Development. 2015;82(7-8):518-29.
11. Stegemann H, Stalder K. Determination of hydroxyproline. Clinica Chimica Acta. 1967;18(2):267-73.
12. Li P, Wu G. Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth. Amino Acids. 2018;50(1):29-38.
13. Ozay R, Keskin E, Balkan MS, et al. Effects of topically applied Contractubex® on epidural fibrosis and axonal regeneration in injured rat sciatic nerve. Turkish Neurosurgery. 2023;33(3):437-46.
14. Seref K, Sonmez K, Gulburun MA, et al. Protective effects of Contractubex® on stricture formation after experimental corrosive esophageal burns in rats. Archives of Medical Research. 2020;51(7):664-9.
15. Garoufalia Z, Papadopetraki A, Karatza E, et al. Insulin-like growth factor-I and wound healing, a potential answer to non-healing wounds: A systematic review of the literature and future perspectives. Biomedical Reports. 2021;15(2):66.
16. León-López A, Morales-Peñaloza A, Martínez-Juárez VM, et al. Hydrolyzed collagen-sources and applications. Molecules. 2019;24(22).
17. Akita S, Akino K, Imaizumi T, et al. Basic fibroblast growth factor accelerates and improves second-degree burn wound healing. Wound Repair and Regeneration. 2008;16(5):635-41.
18. Meng X, Guo M, Geng Z, et al. Effects and mechanism of the Leontopodium alpinum Callus culture extract on blue light damage in human foreskin fibroblasts. Molecules. 2023;28(5).
19. Daniela L, Alla P, Maurelli R, et al. Anti-inflammatory effects of concentrated ethanol extracts of Edelweiss (Leontopodium alpinum Cass.) callus cultures towards human keratinocytes and endothelial cells. Mediators of Inflammation. 2012;2012:498373.
20. Zhang L, Falla TJ. Cosmeceuticals and peptides. Clinics in Dermatology. 2009;27(5):485-94.
21. Pollard JD, Quan S, Kang T, et al. Effects of copper tripeptide on the growth and expression of growth factors by normal and irradiated fibroblasts. JAMA Facial Plastic Surgery. 2005;7(1):27-31.