Evaluation of the immunostimulatory activity of dong trung ha thao banikha in experimental animals
Main Article Content
Abstract
The purpose of this study was to evaluate the immunostimulatory activity of Dong trung ha thao Banikha on animals immunosuppressed by cyclophosphamide (CY). Mice were divided randomly into 5 groups of 10 mice per cage: control, model, positive control (levamisole), Dong trung ha thao Banikha at the dose of 0.77 g/kg/day and Dong trung ha thao Banikha at the dose of 2.31 g/kg/day. Immunosuppression on mice was induced by CY 200 mg/kg intraperitoneally. The indexes consisted of relative organ weight, leukocyte counts, OA induced paw edema, serum IL-2, TNF-α and IgM level and micro-histological images of spleen and thymus. As a result, Dong trung ha thao Banikha at the dose of 0.77 g/kg/day significantly showed immunostimulatory influence; Dong trung ha thao Banikha at the dose of 2.31 g/kg/day only had a tendency to ameliorate the effects of CY on immunosuppressive mice.
Article Details
Keywords
Dong trung ha thao Banikha, immunostimulatory effect, immunosuppression, cyclophosphamide.
References
2. Rang HP, Ritter JM, Flower RJ, Henderson G. Rang and Dale’s pharmacology, eighth edition, Elsiver, UK. 2015.
3. Đào Văn Phan. Dược lý học lâm sàng, Trường Đại học Y Hà Nội, Nhà xuất bản Y học, Hà Nội. 2018.
4. Parnham MJ, Nijkamp FP. Principles of immunopharmacology, second edition. Birkhauser Verlag. 2005;377-389.
5. Đỗ Tất Lợi. Những cây thuốc và vị thuốc Việt Nam, Nhà xuất bản Y học, Hà Nội. 2015.
6. Jung S., Jung E., Choi E., Sin H., Ha K., Chae S. Immunomodulatory effects of a mycelium extract of Cordyceps (Paecilomyces hepiali; CBG-CS-2): a randomized and double-blind clinical trial. BMC Complement Altern Med. 2019;19:77 .
7. Guangyu Xu, Guangxin Yuan, Liping An, Peige Du, Liya Xie, Hongyu Li, Yu Sheng, Xiao Han. Immunomodulatory mechanism of Cordyceps militaris polypeptide through regulating gene Hist1h2bp, Ctsg, and elane in mice. Pharmacognosy Magazine. 2018;14(56):404-410.
8. Shirani K, Hassani FV, Razavi-Azarkhiavi K, Heidari S, Zanjani BR, Karimi G. Phytotrapy of cyclophosphamide-induced immunosuppression. Environ Toxicol Pharmacol. 2015;39(3):1262-1275.
9. Gupta M. Levamisole: A multi-faceted drug in dermatology. Indian J Dermatol Venereol Leprol. 2016;82(2):230-236.
10. Văn Đình Hoa. Sinh lý bệnh và miễn dịch, Phần Sinh lý bệnh học, Trường Đại học Y Hà Nội, Nhà xuất bản Y học, Hà Nội. 2019.
11. Cavalcanti YV, Brelaz MC, Neves JK, Ferraz JC, Pereira VR. Role of TNF-alpha, IFN-gamma, and IL-10 in the development of pulmonary tuberculosis. Pulm Med. 2012;1-10.
12. Sirko A, Vaněk T, Góra-Sochacka A, Redkiewicz P. Recombinant Cytokines from Plants. Int J Mol Sci. 2011;12(6):3536-3552.
13. Shin S, Kwon J, Lee S, Kong H, Lee S, Lee CK, Cho K, Ha NJ, Kim K. Immunostimulatory Effects of Cordyceps militaris on Macrophages through the Enhanced Production of Cytokines via the Activation of NF-kappaB. Immune Netw. 2010;10(2):55-63.
14. Shin JS, Chung SH, Lee WS, Lee JY, Kim JL, Lee KT. Immunostimulatory effects of cordycepin-enriched WIB-801CE from Cordyceps militaris in splenocytes and cyclophosphamide-induced immunosuppressed mice. Phytotherapy research. 2018;32:132–139.
15. Lin Z. Cellular and Molecular Mechanisms of Immuno-modulation by Ganoderma lucidum. J Pharmacol Sci. 2005;99:144 – 153.
16. Gao J, Zhang HJ, Yu SH, Wu SG, Yoon I, Quigley J, Gao YP, Qi GH. Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poult Sci. 2008; 87(7):1377-84.
17. Park SY, Kim HB, Kim JH, Lee JM, Kim SR, Shin HS, Yi TH. Immunostimulatory effect of fermented red ginseng in the mouse model. Prev Nutr Food Sci. 2014;19(1):10-8..