21. Evaluation of anti-hemorrhoidal activity of “Tri 01” hard capsule on experimental model of hemorrhoids in wistar in rats

Tóm tắt

This study evaluated the anti-hemorrhoidal effect of “TRI 01” hard capsule on croton oil-induced hemorrhoids model in rats. Hemorrhoids were induced by applying 6% croton oil preparation in the ano-rectal region. Then, rats were randomly assigned to groups. For the two “TRI 01” groups, “TRI 01” was orally administered at doses of 2.64 and 7.92 g/kg per day for seven days, respectively. Daflon (360 mg/kg per day) was used as reference anti-hemorrhoidal drug. Weighting of ano-rectal tissue, calculation of ano-rectal coefficient, and histology of ano-rectal tissue were conducted two hours after the last administration on the seventh day. The results indicated that treatment using “TRI 01” hard capsule significantly decreased the weight of ano-rectal tissue and the rectoanal coefficient compared to the vehicle-treated hemorrhoid group. Histological observation revealed that TRI 01-treated animals had lesser degree of inflammatory cells, degenerative changes congestion, edema along with lesser degree of necrosis in the mucosal epithelium of ano-rectal tissue as compared to croton-induced hemorrhoid group. Our study demonstrated that “TRI 01” at doses of 2.64 and 7.92 g/kg had anti-hemorrhoidal activity on croton oil-induced hemorrhoids model in rats.