Evaluation of embryo formation efficiency from Rescue IVM immature oocytes
Main Article Content
Abstract
A retrospective descriptive study was conducted to evaluate the embryo development potential of immature oocytes matured through rescue in vitro maturation (rescue IVM). The study included 207 women undergoing in vitro fertilization (IVF) with a total of 2,231 oocytes (1,514 mature and 717 immature) at the Infertility and Medical Sexology Center, Hanoi General Hospital, from January 2024 to December 2024. The in vivo maturation rate was 67.13%, while the proportion of immature oocytes was 32.87% (MI: 27.46% and GV: 5.41%). The maturation rate of immature oocytes (MI, GV) after overnight culture was 87.22%. When comparing embryos derived from denuded MII oocytes and those from rescue IVM MII oocytes, the fertilization rates were similar between the two groups (89.01% and 86.12%, respectively); however, the blastocyst development rate was significantly lower in the rescue IVM MII group compared to the in vivo matured MII group (11.59% vs. 62.79%). No significant correlation was observed between age or total FSH dose and either the rate of mature oocytes or the rate of oocytes maturing after overnight culture (p > 0.05). Although the fertilization rate of rescue IVM MII oocytes was high and comparable to that of normally matured oocytes, their blastocyst development rate was low. Therefore, the indication for overnight culture of immature oocytes for rescue IVM should be individualized for each case.
Article Details
Keywords
Rescue in vitro maturation, immature oocyte maturation, fertilization rate, blastocyst development rate
References
2. Mostinckx L, Segers I, Belva F, et al. Obstetric and neonatal outcome of ART in patients with polycystic ovary syndrome: IVM of oocytes versus controlled ovarian stimulation. Human Reproduction. 2019; 34(8): 1595-1607. doi:10.1093/humrep/dez086.
3. Steward RG, Lan L, Shah AA, et al. Oocyte number as a predictor for ovarian hyperstimulation syndrome and live birth: an analysis of 256,381 in vitro fertilization cycles. Fertility and Sterility. 2014; 101(4): 967-973. doi:10.1016/j.fertnstert.2013.12.026.
4. Magnusson Å, Källen K, Thurin-Kjellberg A, Bergh C. The number of oocytes retrieved during IVF: a balance between efficacy and safety. Hum Reprod. 2018; 33(1): 58-64. doi:10.1093/humrep/dex334.
5. Polyzos NP, Sunkara SK. Sub-optimal responders following controlled ovarian stimulation: an overlooked group? Hum Reprod. 2015; 30(9): 2005-2008. doi:10.1093/humrep/dev149.
6. Ho VNA, Braam SC, Pham TD, Mol BW, Vuong LN. The effectiveness and safety of in vitro maturation of oocytes versus in vitro fertilization in women with a high antral follicle count. Hum Reprod. 2019; 34(6): 1055-1064. doi:10.1093/humrep/dez060.
7. ESHRE Special Interest Group of Embryology, Alpha Scientists in Reproductive Medicine. The Vienna consensus: report of an expert meeting on the development of art laboratory performance indicators. Human Reproduction Open. 2017; 2017(2): hox011. doi:10.1093/hropen/hox011.
8. Lee HJ, Jee BC, Suh CS, Kim SH, Moon SY. Oocyte Maturity in Relation to Woman’s Age in In Vitro Fertilization Cycles Stimulated by Single Regimen. Yonsei Med J. 2012; 53(1): 181. doi:10.3349/ymj.2012.53.1.181.
9. Cha KY, Chian RC. Maturation in vitro of immature human oocytes for clinical use. Hum Reprod Update. 1998; 4(2): 103-120. doi:10.1093/humupd/4.2.103.
10. Qin DY, Jiang HH, Yao QY, et al. Rescue in vitro maturation may increase the pregnancy outcomes among women undergoing intracytoplasmic sperm injection. Front Endocrinol. 2022; 13. doi:10.3389/fendo.2022.1047571.
11. Vincentiis SD, Martino ED, Buffone MG, Brugo-Olmedo S. Use of metaphase I oocytes matured in vitro is associated with embryo multinucleation. Fertility and Sterility. 2013; 99(2): 414-421.e4. doi:10.1016/j.fertnstert.2012.10.028.
12. Reichman DE, Politch J, Ginsburg ES, Racowsky C. Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes. J Assist Reprod Genet. 2010; 27(7): 347-356. doi:10.1007/s10815-010-9416-5.
13. Martin-Palomino Olid N, García D, Rodríguez A, Vassena R. Could fertility clinics offer a sizable improvement of live birth rates by maturing post-GVBD oocytes in vitro? J Assist Reprod Genet. 2019; 36(9): 1927-1934. doi:10.1007/s10815-019-01540-8.
14. Jie H, Zhao M, Alqawasmeh OAM, et al. In vitro rescue immature oocytes - a literature review. Human Fertility. 2022; 25(4): 640-650. doi:10.1080/14647273.2021.1876932.