53. Hoạt tính chống viêm in vitro và in vivo của cao chiết ethyl acetate từ vỏ thân cây núc nác (Oroxylum indicum)
Nội dung chính của bài viết
Tóm tắt
Nghiên cứu này đã khảo sát đặc tính kháng viêm của cao chiết ethyl acetate (EtOAc) từ vỏ thân núc nác (Oroxylum indicum). Tác dụng kháng viêm được đánh giá thông qua hiệu quả ức chế sản sinh nitric oxide (NO) ở đại thực bào RAW 264.7 được kích thích bằng lipopolysaccharid và mô hình viêm màng bụng do carrageenan gây ra trên chuột cống. Cao chiết EtOAc cho thấy khả năng làm giảm đáng kể sản xuất NO in vitro và thể tích dịch tiết phúc mạc cũng như số lượng bạch cầu trong dịch tiết phúc mạc in vivo theo cách phụ thuộc liều. Nhìn chung, kết quả của nghiên cứu này đã chứng minh đặc tính kháng viêm của chiết xuất thử nghiệm trên cả in vitro và in vivo. Do đó, cao chiết EtOAc từ vỏ thân núc nác có thể được sử dụng làm nguồn dược phẩm chức năng tiềm năng để phát triển các thực phẩm bổ sung sức khỏe nhằm hỗ trợ kiểm soát tình trạng viêm.
Chi tiết bài viết
Từ khóa
Núc nác, viêm, RAW 264.7, carrageenan, lipopolysaccharid, chuột cống
Tài liệu tham khảo
1. Megha KB, Joseph X, Akhil V, Mohanan PV. Cascade of immune mechanism and consequences of inflammatory disorders. Phytomedicine. 2021; 91: 153712. doi: 10.1016/j.phymed.2021.153712.
2. Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee MNM. The crucial roles of inflammatory mediators in inflammation: A review. Vet World. 2018; 11(5): 627-635. doi: 10.14202/vetworld.2018.627-635.
3. Stone WL, Basit H, Zubair M, et al. Pathology, Inflammation. [Updated 2024 Aug 11]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK534820/.
4. Sinibaldi S. Anti-inflammatory Drugs: Glucocorticoids. In: Esquinas, A.M., Mina, B., Spadaro, S., Perrotta, D., De Sanctis, F. (eds) Pharmacology in Noninvasive Ventilation. Noninvasive Ventilation. The Essentials. Springer, Cham. 2023. https://doi.org/10.1007/978-3-031-44626-9_28.
5. Ghlichloo I, Gerriets V. Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) [Updated 2023 May 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK547742/.
6. Hao DC, Xiao PG. Pharmaceutical resource discovery from traditional medicinal plants: Pharmacophylogeny and pharmacophylogenomics. Chin Herb Med. 2020; 12(2): 104-117. doi: 10.1016/j.chmed.2020.03.002.
7. Nasim N, Sandeep IS, Mohanty S. Plant-derived natural products for drug discovery: current approaches and prospects. Nucleus (Calcutta). 2022; 65(3): 399-411. doi: 10.1007/s13237-022-00405-3.
8. Dinda B, SilSarma I, Dinda M, Rudrapaul P. Oroxylum indicum (L.) Kurz, an important Asian traditional medicine: from traditional uses to scientific data for its commercial exploitation. J Ethnopharmacol. 2015; 161:255-78. doi: 10.1016/j.jep.2014.12.027.
9. Harminder, Singh V, Chaudhary AK. A Review on the Taxonomy, Ethnobotany, Chemistry and Pharmacology of Oroxylum indicum Vent. Indian J Pharm Sci. 2011; 73(5): 483-90. doi: 10.4103/0250-474X.98981.
10. Süntar I. Importance of ethnopharmacological studies in drug discovery: role of medicinal plants. Phytochem Rev. 2020; 19: 1199-1209. doi: 10.1007/s11101-019-09629-9.
11. ISO. Biological evaluation of medical devices, Part 5: Tests for in vitro cytotoxicity. ISO 10993-5. Geneva: ISO; 2009.
12. Möller MN, Rios N, Trujillo M, Radi R, Denicola A, Alvarez B. Detection and quantification of nitric oxide-derived oxidants in biological systems. J Biol Chem. 2019; 294(40): 14776-14802. doi: 10.1074/jbc.REV119.006136.
13. Facchin BM, Dos Reis GO, Vieira GN, Mohr ETB, da Rosa JS, et al. Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis. Inflamm Res. 2022; 71(7-8): 741-758. doi: 10.1007/s00011-022-01584-0.
14. Csonka C, Páli T, Bencsik P, Görbe A, Ferdinandy P, Csont T. Measurement of NO in biological samples. Br J Pharmacol. 2015; 172(6): 1620-32. doi: 10.1111/bph.12832.
15. Griswold DE, Marshall PJ, Webb EF, Godfrey R, Newton J Jr, et al. SK&F 86002: a structurally novel anti-inflammatory agent that inhibits lipoxygenase- and cyclooxygenase-mediated metabolism of arachidonic acid. Biochem Pharmacol. 1987; 36(20): 3463-70. doi: 10.1016/0006-2952(87)90327-3.
16. Pahwa R, Goyal A, Jialal I. Chronic Inflammation. [Updated 2023 Aug 7]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK493173/.
17. Vb Furman D, Campisi J, Verdin E et al. Chronic inflammation in the etiology of disease across the life span. Nat Med. 2019; 25: 1822–1832. doi: 10.1038/s41591-019-0675-0.
18. Tian Y, Zhou S, Takeda R, Okazaki K, Sekita M, Sakamoto K. Anti-inflammatory activities of amber extract in lipopolysaccharide-induced RAW 264.7 macrophages. Biomed Pharmacother. 2021; 141: 111854. doi: 10.1016/j.biopha.2021.111854.
19. Sharma JN, Al-Omran A, Parvathy SS. Role of nitric oxide in inflammatory diseases. Inflammopharmacology. 2007; 15(6): 252-259. doi: 10.1007/s10787-007-0013-x.
20. Mairuae N, Cheepsunthor P, Buranrat B. Antioxidant and anti-inflammatory activities of Oroxylum indicum Kurz (L.) fruit extract in lipopolysaccharide-stimulated BV2 microglial cells. Tropical Journal of Pharmaceutical Research. 2021; 20(4): 833-838. doi: 10.4314/tjpr.v20i4.25.
21. Mairuae N, Buranrat B, Yannasithinon S, Cheepsunthor P. Oroxylum indicum Kurz (L) leaf extract exerted antioxidant and anti-inflammatory effects on LPS-stimulated BV2 microglial cells. Tropical Journal of Pharmaceutical Research. 2024; 23(9): 1409-1415. doi: 10.4314/tjpr.v23i9.2.
22. Dunkhunthod B, Talabnin C, Murphy M, Thumanu K, Sittisart P, et al. Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages. Evid Based Complement Alternat Med. 2020; 2020: 7436920. doi: 10.1155/2020/7436920.
23. Sousa-Neto BP, Cunha FVM, Nunes DB, Gomes BS, Amorim LV, et al. Anti-Inflammatory and Antioxidant Effects of the Indole-Derived N-Salicyloyltryptamine on Peritonitis and Joint Disability Induced by Carrageenan in Rodents. Evid Based Complement Alternat Med. 2022; 2022: 5524107. doi: 10.1155/2022/5524107.
24. Lalrinzuali K, Vabeiryureilai M, Jagetia GC. Investigation of the Anti-Inflammatory and Analgesic Activities of Ethanol Extract of Stem Bark of Sonapatha Oroxylum indicum In Vivo. Int J Inflam. 2016; 2016: 8247014. doi: 10.1155/2016/8247014.
25. Pathak BK, Palandurkar KM, Singh M, Trigunayat A, Singh A, et al. Evaluation of In vivo Analgesic and Anti-inflammatory Activity of Oroxyulum indicum, Baicalein, Chrysin with Phytochemical Analysis and Molecular Docking Study. Pharmacogn J. 2023; 15(5): 811-822.
26. Lalrinzuali K, Vabeiryureilai M, Jagetia GC, Lalawmpuii PC. Free radical scavenging and antioxidant potential of different extracts of Oroxylum indicum in vitro. Advances in Biomedicine and Pharmacy. 2015; 2(3): 120-130. doi: 10.19046/abp.v02i03.02.
27. Liu X, Wang T, Liu X, Cai L, Qi J, et al. Biochanin A protects lipopolysaccharide/D-galactosamine-induced acute liver injury in mice by activating the Nrf2 pathway and inhibiting NLRP3 inflammasome activation. Int Immunopharmacol. 2016; 38: 324-31. doi: 10.1016/j.intimp.2016.06.009.
28. Yao Y, Chen L, Xiao J, Wang C, Jiang W, et al. Chrysin protects against focal cerebral ischemia/reperfusion injury in mice through attenuation of oxidative stress and inflammation. Int J Mol Sci. 2014; 15(11): 20913-26. doi: 10.3390/ijms151120913.
29. Hu Z, Guan Y, Hu W, Xu Z, Ishfaq M. An overview of pharmacological activities of baicalin and its aglycone baicalein: New insights into molecular mechanisms and signaling pathways. Iran J Basic Med Sci. 2022; 25(1): 14-26. doi: 10.22038/IJBMS.2022.60380.13381.
30. Sajeev A, Hegde M, Girisa S, Devanarayanan TN, Alqahtani MS, et al. Oroxylin A: A Promising Flavonoid for Prevention and Treatment of Chronic Diseases. Biomolecules. 2022; 12(9): 1185. doi: 10.3390/biom12091185.
2. Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee MNM. The crucial roles of inflammatory mediators in inflammation: A review. Vet World. 2018; 11(5): 627-635. doi: 10.14202/vetworld.2018.627-635.
3. Stone WL, Basit H, Zubair M, et al. Pathology, Inflammation. [Updated 2024 Aug 11]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK534820/.
4. Sinibaldi S. Anti-inflammatory Drugs: Glucocorticoids. In: Esquinas, A.M., Mina, B., Spadaro, S., Perrotta, D., De Sanctis, F. (eds) Pharmacology in Noninvasive Ventilation. Noninvasive Ventilation. The Essentials. Springer, Cham. 2023. https://doi.org/10.1007/978-3-031-44626-9_28.
5. Ghlichloo I, Gerriets V. Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) [Updated 2023 May 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK547742/.
6. Hao DC, Xiao PG. Pharmaceutical resource discovery from traditional medicinal plants: Pharmacophylogeny and pharmacophylogenomics. Chin Herb Med. 2020; 12(2): 104-117. doi: 10.1016/j.chmed.2020.03.002.
7. Nasim N, Sandeep IS, Mohanty S. Plant-derived natural products for drug discovery: current approaches and prospects. Nucleus (Calcutta). 2022; 65(3): 399-411. doi: 10.1007/s13237-022-00405-3.
8. Dinda B, SilSarma I, Dinda M, Rudrapaul P. Oroxylum indicum (L.) Kurz, an important Asian traditional medicine: from traditional uses to scientific data for its commercial exploitation. J Ethnopharmacol. 2015; 161:255-78. doi: 10.1016/j.jep.2014.12.027.
9. Harminder, Singh V, Chaudhary AK. A Review on the Taxonomy, Ethnobotany, Chemistry and Pharmacology of Oroxylum indicum Vent. Indian J Pharm Sci. 2011; 73(5): 483-90. doi: 10.4103/0250-474X.98981.
10. Süntar I. Importance of ethnopharmacological studies in drug discovery: role of medicinal plants. Phytochem Rev. 2020; 19: 1199-1209. doi: 10.1007/s11101-019-09629-9.
11. ISO. Biological evaluation of medical devices, Part 5: Tests for in vitro cytotoxicity. ISO 10993-5. Geneva: ISO; 2009.
12. Möller MN, Rios N, Trujillo M, Radi R, Denicola A, Alvarez B. Detection and quantification of nitric oxide-derived oxidants in biological systems. J Biol Chem. 2019; 294(40): 14776-14802. doi: 10.1074/jbc.REV119.006136.
13. Facchin BM, Dos Reis GO, Vieira GN, Mohr ETB, da Rosa JS, et al. Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis. Inflamm Res. 2022; 71(7-8): 741-758. doi: 10.1007/s00011-022-01584-0.
14. Csonka C, Páli T, Bencsik P, Görbe A, Ferdinandy P, Csont T. Measurement of NO in biological samples. Br J Pharmacol. 2015; 172(6): 1620-32. doi: 10.1111/bph.12832.
15. Griswold DE, Marshall PJ, Webb EF, Godfrey R, Newton J Jr, et al. SK&F 86002: a structurally novel anti-inflammatory agent that inhibits lipoxygenase- and cyclooxygenase-mediated metabolism of arachidonic acid. Biochem Pharmacol. 1987; 36(20): 3463-70. doi: 10.1016/0006-2952(87)90327-3.
16. Pahwa R, Goyal A, Jialal I. Chronic Inflammation. [Updated 2023 Aug 7]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK493173/.
17. Vb Furman D, Campisi J, Verdin E et al. Chronic inflammation in the etiology of disease across the life span. Nat Med. 2019; 25: 1822–1832. doi: 10.1038/s41591-019-0675-0.
18. Tian Y, Zhou S, Takeda R, Okazaki K, Sekita M, Sakamoto K. Anti-inflammatory activities of amber extract in lipopolysaccharide-induced RAW 264.7 macrophages. Biomed Pharmacother. 2021; 141: 111854. doi: 10.1016/j.biopha.2021.111854.
19. Sharma JN, Al-Omran A, Parvathy SS. Role of nitric oxide in inflammatory diseases. Inflammopharmacology. 2007; 15(6): 252-259. doi: 10.1007/s10787-007-0013-x.
20. Mairuae N, Cheepsunthor P, Buranrat B. Antioxidant and anti-inflammatory activities of Oroxylum indicum Kurz (L.) fruit extract in lipopolysaccharide-stimulated BV2 microglial cells. Tropical Journal of Pharmaceutical Research. 2021; 20(4): 833-838. doi: 10.4314/tjpr.v20i4.25.
21. Mairuae N, Buranrat B, Yannasithinon S, Cheepsunthor P. Oroxylum indicum Kurz (L) leaf extract exerted antioxidant and anti-inflammatory effects on LPS-stimulated BV2 microglial cells. Tropical Journal of Pharmaceutical Research. 2024; 23(9): 1409-1415. doi: 10.4314/tjpr.v23i9.2.
22. Dunkhunthod B, Talabnin C, Murphy M, Thumanu K, Sittisart P, et al. Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages. Evid Based Complement Alternat Med. 2020; 2020: 7436920. doi: 10.1155/2020/7436920.
23. Sousa-Neto BP, Cunha FVM, Nunes DB, Gomes BS, Amorim LV, et al. Anti-Inflammatory and Antioxidant Effects of the Indole-Derived N-Salicyloyltryptamine on Peritonitis and Joint Disability Induced by Carrageenan in Rodents. Evid Based Complement Alternat Med. 2022; 2022: 5524107. doi: 10.1155/2022/5524107.
24. Lalrinzuali K, Vabeiryureilai M, Jagetia GC. Investigation of the Anti-Inflammatory and Analgesic Activities of Ethanol Extract of Stem Bark of Sonapatha Oroxylum indicum In Vivo. Int J Inflam. 2016; 2016: 8247014. doi: 10.1155/2016/8247014.
25. Pathak BK, Palandurkar KM, Singh M, Trigunayat A, Singh A, et al. Evaluation of In vivo Analgesic and Anti-inflammatory Activity of Oroxyulum indicum, Baicalein, Chrysin with Phytochemical Analysis and Molecular Docking Study. Pharmacogn J. 2023; 15(5): 811-822.
26. Lalrinzuali K, Vabeiryureilai M, Jagetia GC, Lalawmpuii PC. Free radical scavenging and antioxidant potential of different extracts of Oroxylum indicum in vitro. Advances in Biomedicine and Pharmacy. 2015; 2(3): 120-130. doi: 10.19046/abp.v02i03.02.
27. Liu X, Wang T, Liu X, Cai L, Qi J, et al. Biochanin A protects lipopolysaccharide/D-galactosamine-induced acute liver injury in mice by activating the Nrf2 pathway and inhibiting NLRP3 inflammasome activation. Int Immunopharmacol. 2016; 38: 324-31. doi: 10.1016/j.intimp.2016.06.009.
28. Yao Y, Chen L, Xiao J, Wang C, Jiang W, et al. Chrysin protects against focal cerebral ischemia/reperfusion injury in mice through attenuation of oxidative stress and inflammation. Int J Mol Sci. 2014; 15(11): 20913-26. doi: 10.3390/ijms151120913.
29. Hu Z, Guan Y, Hu W, Xu Z, Ishfaq M. An overview of pharmacological activities of baicalin and its aglycone baicalein: New insights into molecular mechanisms and signaling pathways. Iran J Basic Med Sci. 2022; 25(1): 14-26. doi: 10.22038/IJBMS.2022.60380.13381.
30. Sajeev A, Hegde M, Girisa S, Devanarayanan TN, Alqahtani MS, et al. Oroxylin A: A Promising Flavonoid for Prevention and Treatment of Chronic Diseases. Biomolecules. 2022; 12(9): 1185. doi: 10.3390/biom12091185.