Article Sidebar

PDF (Tiếng Việt)
Published: 2022-08-31
Abstract Views: 324
Views PDF (Tiếng Việt): 314
DOI: https://doi.org/10.52852/tcncyh.v156i8.1000
Issue
Vol. 156 No. 8 (2022)
Section
Các bài báo
How to Cite
Đại, H. V., Nhung, V. P., Tâm, N. T. T. ., Ngọc, L. T. ., Hà, N. H., Thạch, P. N., & Hải, N. T. T. (2022). 2. Association of nasopharyngeal ace2 and tmprss2 expression to severity of COVID-19. Journal of Medical Research, 156(8), 11-19. https://doi.org/10.52852/tcncyh.v156i8.1000

2. Association of nasopharyngeal ace2 and tmprss2 expression to severity of COVID-19

Ha Van Đai1, Vu Phuong Nhung, Nguyen Thi Thanh Tam, Le Thi Ngoc, Nguyen Hai Ha, Pham Ngoc Thach, Nguyen Thi Thanh Hai
1 Bệnh viện Bệnh Nhiệt đới Trung Ương

Main Article Content

Abstract

ACE2 is a cellular membrane-bound receptor enzyme that facilitates the entry of SARS-CoV-2 virus into the cell through binding to the spike (S) protein on their surface. Spike (S) protein is cleaved by cellular transmembrane protease serine 2 (TMPRSS2) at the S1/S2 and S'2 sites that facilitate the process of viral and host membrane fusion and invasion. Thus, the level of expression of these host cell proteins may augment the virus invasion that impacts clinical symptoms. The clinical and paraclinical characteristics of the 297 COVID-19 patients were accessed. Representative 52 nasopharyngeal fluid samples randomly selected from typical severe and mild patients, respectively, were relatively compared for RNA- expression of ACE2 and TMPRSS2 by using realtime-PCR with specific primers. The resuls showed that TMPRSS2 but not ACE2 gene expression in the nasopharyngeal mucosa and some paraclinical indicators such as CRP, PCT, Ferritin, D-Dimer, LDH may predict the severity of COVID-19 patients but further study is required with larger sample sizes.

Article Details

Keywords

COVID-19, ACE2, TMPRSS2, Gene expression

References

1. Cập nhật số ca nhiễm Covid-19 hôm nay mới nhất trên VnExpress. Accessed June 23, 2022. https://vnexpress.net/covid-19/covid-19-viet-nam.
2. Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet Lond Engl. 2020; 395(10223): 497-506. doi:10.1016/S0140-6736(20)30183-5.
3. Dassarma B, Tripathy S, Chabalala M, Matsabisa MG. Challenges in Establishing Vaccine Induced Herd Immunity through Age Specific Community Vaccinations. Aging Dis. 2022; 13(1): 29-36. doi:10.14336/AD.2021.0611.
4. Hoffmann M, Kleine-Weber H, Schroeder S, et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020; 181(2): 271-280.e8. doi:10.1016/j.cell.2020.02.052.
5. TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cells - PubMed. Accessed June 23, 2022. https://pubmed.ncbi.nlm.nih.gov/32703818/.
6. Kuba K, Imai Y, Rao S, et al. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus-induced lung injury. Nat Med. 2005; 11(8): 875-879. doi:10.1038/nm1267.
7. Bao J, Li C, Zhang K, Kang H, Chen W, Gu B. Comparative analysis of laboratory indexes of severe and non-severe patients infected with COVID-19. Clin Chim Acta Int J Clin Chem. 2020; 509: 180-194. doi:10.1016/j.cca.2020.06.009.
8. Pourbagheri-Sigaroodi A, Bashash D, Fateh F, Abolghasemi H. Laboratory findings in COVID-19 diagnosis and prognosis. Clin Chim Acta Int J Clin Chem. 2020; 510: 475-482. doi:10.1016/j.cca.2020.08.019.
9. Smith JC, Sausville EL, Girish V, et al. Cigarette Smoke Exposure and Inflammatory Signaling Increase the Expression of the SARS-CoV-2 Receptor ACE2 in the Respiratory Tract. Dev Cell. 2020; 53(5): 514-529.e3. doi:10.1016/j.devcel.2020.05.012.
10. ACE2 Deficiency Worsens Epicardial Adipose Tissue Inflammation and Cardiac Dysfunction in Response to Diet-Induced Obesity - PubMed. Accessed June 23, 2022. https://pubmed.ncbi.nlm.nih.gov/26224885/.
11. Bénéteau-Burnat B, Baudin B, Morgant G, Baumann FC, Giboudeau J. Serum angiotensin-converting enzyme in healthy and sarcoidotic children: comparison with the reference interval for adults. Clin Chem. 1990; 36(2): 344-346.
12. Hoffmann: Activation of viruses by host proteases - Google Scholar. Accessed June 30, 2021. https://scholar.google.com/scholar_lookup?title=Activation+of+Viruses+by+Host+Proteases&author=M+Hoffmann&author=H+Hofmann-Winkler&author=S+P%C3%B6hlmann&publication_year=2018&.
13. Klenk HD, Garten W. Host cell proteases controlling virus pathogenicity. Trends Microbiol. 1994; 2(2): 39-43. doi:10.1016/0966-842x(94)90123-6.
14. Matsuyama S, Ujike M, Morikawa S, Tashiro M, Taguchi F. Protease-mediated enhancement of severe acute respiratory syndrome coronavirus infection. Proc Natl Acad Sci U S A. 2005; 102(35): 12543-12547. doi:10.1073/pnas.0503203102.
15. Iwata-Yoshikawa N, Okamura T, Shimizu Y, Hasegawa H, Takeda M, Nagata N. TMPRSS2 Contributes to Virus Spread and Immunopathology in the Airways of Murine Models after Coronavirus Infection. J Virol. 2019; 93(6): e01815-18. doi:10.1128/JVI.01815-18.
16. Piva F, Sabanovic B, Cecati M, Giulietti M. Expression and co-expression analyses of TMPRSS2, a key element in COVID-19. Eur J Clin Microbiol Infect Dis Off Publ Eur Soc Clin Microbiol. 2021; 40(2): 451-455. doi:10.1007/s10096-020-04089-y.
17. Bunyavanich S, Do A, Vicencio A. Nasal Gene Expression of Angiotensin-Converting Enzyme 2 in Children and Adults. JAMA. 2020; 323(23): 2427-2429. doi:10.1001/jama.2020.8707.