3. Identification of various mycobacteria strains to the species level by gene sequencing
Main Article Content
Abstract
Despite the close genetic relatedness, members of the genus Mycobacterium differ significantly in morphology, biochemistry, host range and pathogenicity. TBc ID (Becton Dickinson, Sparks, MD) is a rapid and reliable method for MTBC identification in liquid culture assay, however a small minority of MTBC isolates are not detected by TBc ID assay due to none to low concentration of MPT64 in liquid culture; moreover, TBc ID cannot distinguish mycobacteria to species. Identification of mycobacteria isolates to the species level by analysis of several target genes (including 16S rRNA, gyrB, rpoB, hsp65 and ITS) could overcome these disadvantages and accurately differentiate some less common NTM. Overall, there are 105 TBc ID negative strains, including 56 MTBC strains (53.3%) and 49 NTM strains (46.7%). Looking closely to species level identification of all 56 MTBC strains, M. tuberculosis took an important part and solely M. bovis strain was detected. Within 49 NTM strains, the predominant is M. abscessus/ M. chelonae complex, the second in common is MAC and the other 13 NTM species are in smaller portions.
Article Details
Keywords
Mycobacteria, TBc ID, sequencing
References
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