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Published: 2023-01-10
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DOI: https://doi.org/10.52852/tcncyh.v160i12V1.1137
Issue
Vol. 160 No. 12V1 (2022)
Section
Các bài báo
How to Cite
Niê, H. N., Nhung, P. H. ., Châu, T. M. ., Hiếu, V. N. ., & Trường, L. Đức . (2023). 1. Detection of carbapenemase genes in Klebsiella pneumoniae as untyped carbapenemase producer by Phoenix M50. Journal of Medical Research, 160(12V1), 1-7. https://doi.org/10.52852/tcncyh.v160i12V1.1137

1. Detection of carbapenemase genes in Klebsiella pneumoniae as untyped carbapenemase producer by Phoenix M50

H’ Nuong Nie, Pham Hong Nhung, Tran Minh Chau, Vu Ngoc Hieu, Lai Duc Truong

Main Article Content

Abstract

Increasing prevalence of carbapenem resistant Klebsiella pneumoniae is a global concern. Carbapenem resistant in Klebsiella pneumoniae can be mediated by carbapenemase production or by extended-spectrum beta-lactamase or AmpC beta-lactamase production. In this study, carbapenemase-encoding genes including blaKPC, blaNDM, blaOXA48, blaVIM, and blaIMP were detected in 187 carbapenemase producing Klebsiella pneumoniae by multiplex PCR. The most commonly encountered carbapenemase genes were blaKPC (58.3%), blaNDM (21.4%) and blaOXA-48-like (18.7%). The blaVIM and blaIMP genes were not detected in any of these isolates. 53 of the 187 isolates harboured multiple carbapenemase genes such as blaKPC+blaNDM, blaKPC+blaOXA48-like, blaNDM+blaOXA-48-like, and blaKPC+blaNDM+blaOXA-48-like. Most of these isolates were highly resistant to many commonly used antibiotics.

Article Details

Keywords

carbapenemase, antibiotic resistance, Klebsiella pneumoniae, ARM genes

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