14. Initial results of sperm preparation using density mini-gradient and single-layer centrifugation for oligospermia samples
Main Article Content
Abstract
This study compared sperm recovery rate and sperm DNA fragmentation index (DFI) by density mini-gradient and single layer centrifugation for intrauterine insemination (IUI). A total of 30 semen samples with concentration less than 15 million/ml were prepared by 3 methods: mini-gradient, 90% single lower layer, and 45% single upper layer. Total sperm motility, sperm recovery rate were compared before and after processing for each methods and also after processing among the three methods groups. Our data showed that sperm concentration of 45% single upper layer was significantly higher than the other two groups (p < 0.05). Motile sperm rate in the 45% single layer group was significantly lower than the other groups (p < 0.05). Motile sperm recovery rate of the 45% single layer group was higher than the other groups. All of these methods are viable options for preparing sperm for IUI. A suitable method can be chosen depending on initial sperm quality so that motile sperm with full DNA integrity are collected.
Keywords: Sperm preparation, IUI, mini-gradient, single layer centrifugation
Article Details
Keywords
Sperm preparation, IUI, mini-gradient, single layer centrifugation
References
2. Jeong M, Kim SK, Kim H, Lee JR, Jee BC, Kim SH. Predictive value of sperm motility before and after preparation for the pregnancy outcomes of intrauterine insemination. Clin Exp Reprod Med. Sep 2021; 48(3): 255-261. doi: 10.5653/cerm.2021.04469.
3. Hernandez-Silva G, Lopez-Torres AS, Maldonado-Rosas I, et al. Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation. World J Mens Health. Oct 2021; 39(4): 740-749. doi: 10.5534/wjmh.200115.
4. Wiweko B, Utami P. Predictive value of sperm deoxyribonucleic acid (DNA) fragmentation index in male infertility. Basic Clin Androl. 2017; 27: 1. doi: 10.1186/s12610-016-0046-3.
5. Starosta A, Gordon CE, Hornstein MD. Predictive factors for intrauterine insemination outcomes: A review. Fertil Res Pract. Dec 11 2020; 6(1): 23. doi: 10.1186/s40738-020-00092-1.
6. Facio CL, Previato LF, Machado-Paula LA, Matheus PC, Araujo EF. Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation. JBRA Assist Reprod. Dec 1 2016; 20(4): 206-211. doi: 10.5935/1518-0557.20160040.
7. Lemmens L, Kos S, Beijer C, et al. Techniques used for IUI: Is it time for a change?. Hum Reprod. Sep 1 2017; 32(9): 1835-1845. doi: 10.1093/humrep/dex223.
8. Ord T, Patrizio P, Marello E, Balmaceda JP, Asch RH. Mini-Percoll: A new method of semen preparation for IVF in severe male factor infertility. Hum Reprod. Nov 1990; 5(8): 987-9. doi: 10.1093/oxfordjournals.humrep.a137233.
9. Yu W, Du N, Gu Y, Yan J, Hou W. Specific Ion Effects on the colloidal stability of layered double hydroxide single-layer nanosheets. Langmuir. Jun 16 2020; 36(23): 6557-6568. doi: 10.1021/acs.langmuir.0c01089.
10. Du Y. Single-layer density gradient centrìugation is a simple and effective sperm preparation approach in decreasing the incidence of the contamination originated in the potential pathogens in human sperm samples during ivf practice. Fertility and Sterility. September 2020;114(3):137. doi: 10.1016/j.fertnstert.2020.08.406.
11. Gloria A, Carluccio A, Wegher L, Robbe D, Befacchia G, Contri A. Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates. J Anim Sci Biotechnol. 2016; 7:30. doi: 10.1186/s40104-016-0088-6.