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Published: 2023-01-31
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DOI: https://doi.org/10.52852/tcncyh.v162i1.1339
Issue
Vol. 162 No. 1 (2023)
Section
Các bài báo
How to Cite
Thị Như Lê, T., Ngọc Ánh, T. . ., & Vũ Trung, N. . (2023). 15. Determination of mic and levofloxacin resistance mutation of helicobacter pylori in patients with gastritis and peptic ulcer disease in Tien Giang. Journal of Medical Research, 162(1), 129-137. https://doi.org/10.52852/tcncyh.v162i1.1339

15. Determination of mic and levofloxacin resistance mutation of helicobacter pylori in patients with gastritis and peptic ulcer disease in Tien Giang

Tran Thi Nhu Le, Tran Ngoc Anh, Nguyen Vu Trung

Main Article Content

Abstract

Antibiotic resistance in Helicobacter pylori is increasingly more common and can lead to treatment failure. This study used the Etest and Sanger sequencing techniques to determine the minimum inhibitory concentration (MIC) and mutant forms of Helicobacter pylori in patients with gastritis and peptic ulcer disease in Tien Giang province, Vietnam. The relationship between the MIC and levofloxacin-resistant mutant forms of H. pylori was also explored. Results showed that GyrA mutations accounted for 60% of the total 65 samples studied. Among these mutations, 23.1% (9/39) of LEV-sensitive samples with a MIC of LEV ≤ 1 µg/ml had mutations at codon positions P188L, R190Q, M191I, Y81C, P187S, D91T, D108E, R95K, G111A, and S185Y; and 30/39 (66.9%) samples with MIC > 1 µg/ml were found to have mutations at positions N87K, D91N/G, A55S, M191I, Y28F/I, V107F, A27L, V199G, S29F, M30L, G111L, R42K, V65S, A66L, S101L, M102A, N112I, R95G/K, D108V/E, and A94G. This study suggested that mutations in GyrA at codon positions N87K, D91N/G/T, and A55S of H. pylori were associated with increased MIC concentration of levofloxacin. The related mutations M191I, Y28F/I, V107F, A27L, V199G, S29F, M30L, G111L, R42K, V65S, A66L, S101L, M102A, N112I, R95G/K, D108V/E, and A94G on GyrA were not identified. Further research on the LEV resistance of H. pylori in Tien Giang is needed.

Article Details

Keywords

Helicobacter pylori, levofloxacin, GyrA

References

1. Tonolini M, Ierardi AM, Bracchi E, Magistrelli P, Vella A, Carrafiello GJIii. Non-perforated peptic ulcer disease: multidetector CT findings, complications, and differential diagnosis. 2017; 8(5): 455-469.
2. Hương VTT, Chính VQ, Châu NTH, Cư LVJTcKhvCnTl. Kết quả nghiên cứu thực trạng và các giải pháp quản lý môi trường trong chăn nuôi hộ gia đình và trang trại nhỏ ở một số tỉnh Miền Bắc. 2013; 8.
3. Hoàng Trọng Thảng, Hoàng Thị Tú Anh. Đánh giá hiệu quả của phác đồ Rabeprazole-Levofloxacin- Tinidazole trên bệnh nhân viêm dạ dày mạn nhiễm Helicobacter pylori. Tạp chí Khoa học tiêu hóa Việt Nam. 2015; IX(41): tr.115-120.
4. Miftahussurur M, Yamaoka YJM. Appropriate first-line regimens to combat Helicobacter pylori antibiotic resistance: an Asian perspective. 2015; 20(4): 6068-6092.
5. Trần Thiện Trung, Trần Anh Minh, Nguyễn Tuấn Anh. Nghiên cứu tỷ lệ đột biến kháng thuốc clarithromycin và levofloxacin của H. Pylori bằng giải trình tự gen. Tạp chí khoa học tiêu hóa Việt Nam. 2017; IX(49): 3074-3082.
6. Tuan VP, Narith D, Tshibangu-Kabamba E, et al. A next-generation sequencing-based approach to identify genetic determinants of antibiotic resistance in Cambodian Helicobacter pylori clinical isolates. 2019; 8(6): 858.
7. Alarcon T, Urruzuno P, Martinez MJ, et al. Antimicrobial susceptibility of 6 antimicrobial agents in Helicobacter pylori clinical isolates by using EUCAST breakpoints compared with previously used breakpoints. Enfermedades infecciosas y microbiologia clinica. May 2017; 35(5): 278-282. doi: 10.1016/j.eimc.2016.02.010.
8. Bińkowska A, Biernat MM, Łaczmański Ł, Gościniak GJFim. Molecular patterns of resistance among Helicobacter pylori strains in south-western Poland. 2018; 9: 3154.
9. Lok C-H, Zhu D, Wang J, et al. Phenotype and Molecular Detection of Clarithromycin and Levofloxacin Resistance in Helicobacter pylori Clinical Isolates in Beijing. 2020; 13: 2145.
10. Camorlinga-Ponce M, Gómez-Delgado A, Aguilar-Zamora E, et al. Phenotypic and genotypic antibiotic resistance patterns in Helicobacter pylori strains from ethnically diverse population in Mexico. 2021; 10: 539115.
11. Domanovich-Asor T, Motro Y, Khalfin B, Craddock HA, Peretz A, Moran-Gilad JJM. Genomic analysis of antimicrobial resistance genotype-to-phenotype agreement in Helicobacter pylori. 2020; 9(1): 2.
12. Mascellino MT, Oliva A, Miele MC, De Angelis M, Bruno G, Severi CJA. Secondary antibiotic resistance, correlation between genotypic and phenotypic methods and treatment in Helicobacter pylori infected patients: A retrospective study. 2020; 9(9): 549.
13. Wang Y-h, Gong X-l, Liu D-w, et al. Characteristics of Helicobacter pylori heteroresistance in gastric biopsies and its clinical relevance. 2022: 1409.
14. Farzi N, Yadegar A, Sadeghi A, et al. High prevalence of antibiotic resistance in Iranian Helicobacter pylori isolates: importance of functional and mutational analysis of resistance genes and virulence genotyping. 2019; 8(11): 2004.
15. Arslan N, Yılmaz Ö, Demiray-Gürbüz EJWJoG. Importance of antimicrobial susceptibility testing for the management of eradication in Helicobacter pylori infection. 2017; 23(16): 2854.
16. Tshibangu-Kabamba E, Ngoma-Kisoko PdJ, Tuan VP, et al. Next-generation sequencing of the whole bacterial genome for tracking molecular insight into the broad-spectrum antimicrobial resistance of Helicobacter pylori clinical isolates from the Democratic Republic of Congo. 2020; 8(6): 887.
17. Zhou Y, Zhong Z, Hu S, et al. A Survey of Helicobacter pylori Antibiotic-Resistant Genotypes and Strain Lineages by Whole-Genome Sequencing in China. 2022: e02188-21.