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Published: 2023-01-31
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DOI: https://doi.org/10.52852/tcncyh.v162i1.1353
Issue
Vol. 162 No. 1 (2023)
Section
Các bài báo
How to Cite
Thị An Hà, H., Vũ Trung, N. ., Hà Thanh, N. ., Duy Thái, P., Thị Lan Hương , N. ., Thị Mai Hưng, T., & Huy Hoàng, T. (2023). 18. Characteristics of fluoroquinolone resistant enterococcus faecalis isolates obtained from humans, animals and food. Journal of Medical Research, 162(1), 157-168. https://doi.org/10.52852/tcncyh.v162i1.1353

18. Characteristics of fluoroquinolone resistant enterococcus faecalis isolates obtained from humans, animals and food

Hoang Thi An Ha, Nguyen Vu Trung, Nguyen Ha Thanh, Pham Duy Thai, Nguyen Thi Lan Huong, Tran Thi Mai Hung, Tran Huy Hoang

Main Article Content

Abstract

Enterococcus faecalis is a common opportunistic pathogen in hospitals and the community. Due to its prevalence in variety of environments and resistance to many antibiotics, E. faecalis is an important bacterium to study. Fluoroquinolone is a group of antibiotics that is widely used in clinical practice, especially for treating urinary tract infections caused by gram-positive E. faecalis. The use of fluoroquinolones in hospitals and in livestock has increased the likelihood of resistant colonies which can be transmitted. This study assessed the characteristics of antibiotic resistance of E. faecalis in 466 strains of E. faecalis isolated from human, chicken, flies and food.  The rate of resistance to ciprofloxacin was 19.5% and to levofloxacin was 17.6%. The susceptibility test of fluoroquinolone-resistant strains to other antibiotics showed high rates of resistance to macrolides, phenicol, tetracycline (> 90%), high level aminoglycoside (27.5% - 53.9%) and linezolid (63.7%).  Resistance to penicillin and ampicillin were rare. Genes associated with antibiotic resistance were gyrA (present in 14.4% of ciprofloxacin-resistant strains and 18.5% of levofloxacin-resistant strains), emeA, ermA, ermB, cat, fexA, tetM, tetL and tetK. All linezolid-resistant strains carried optrA but poxtA is absent. High-level aminoglycoside-resistant strains identified contained aac(6′)-Ie/aph(2′′)-Ia, aph(3′)-IIIa, ant(6')-Ia. The gene responsible for the exfflus pumps, emeA, was widely present in E. faecalis strains but did not have a statistically significant association with the level of fluoroquinolone resistance.

Article Details

Keywords

E. faecalis, fluoroquinolone, antibiotic resistance

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