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Published: 2023-01-31
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DOI: https://doi.org/10.52852/tcncyh.v162i1.1353
Issue
Vol. 162 No. 1 (2023)
Section
Các bài báo
How to Cite
Thị An Hà, H., Vũ Trung, N. ., Hà Thanh, N. ., Duy Thái, P., Thị Lan Hương , N. ., Thị Mai Hưng, T., & Huy Hoàng, T. (2023). 18. Characteristics of fluoroquinolone resistant enterococcus faecalis isolates obtained from humans, animals and food. Journal of Medical Research, 162(1), 157-168. https://doi.org/10.52852/tcncyh.v162i1.1353

18. Characteristics of fluoroquinolone resistant enterococcus faecalis isolates obtained from humans, animals and food

Hoang Thi An Ha, Nguyen Vu Trung, Nguyen Ha Thanh, Pham Duy Thai, Nguyen Thi Lan Huong, Tran Thi Mai Hung, Tran Huy Hoang

Main Article Content

Abstract

Enterococcus faecalis is a common opportunistic pathogen in hospitals and the community. Due to its prevalence in variety of environments and resistance to many antibiotics, E. faecalis is an important bacterium to study. Fluoroquinolone is a group of antibiotics that is widely used in clinical practice, especially for treating urinary tract infections caused by gram-positive E. faecalis. The use of fluoroquinolones in hospitals and in livestock has increased the likelihood of resistant colonies which can be transmitted. This study assessed the characteristics of antibiotic resistance of E. faecalis in 466 strains of E. faecalis isolated from human, chicken, flies and food.  The rate of resistance to ciprofloxacin was 19.5% and to levofloxacin was 17.6%. The susceptibility test of fluoroquinolone-resistant strains to other antibiotics showed high rates of resistance to macrolides, phenicol, tetracycline (> 90%), high level aminoglycoside (27.5% - 53.9%) and linezolid (63.7%).  Resistance to penicillin and ampicillin were rare. Genes associated with antibiotic resistance were gyrA (present in 14.4% of ciprofloxacin-resistant strains and 18.5% of levofloxacin-resistant strains), emeA, ermA, ermB, cat, fexA, tetM, tetL and tetK. All linezolid-resistant strains carried optrA but poxtA is absent. High-level aminoglycoside-resistant strains identified contained aac(6′)-Ie/aph(2′′)-Ia, aph(3′)-IIIa, ant(6')-Ia. The gene responsible for the exfflus pumps, emeA, was widely present in E. faecalis strains but did not have a statistically significant association with the level of fluoroquinolone resistance.

Article Details

Keywords

E. faecalis, fluoroquinolone, antibiotic resistance

References

1. Diane S. Daniel SML, Han M. Gan et al. Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia. Journal of Infection and Public Health. 2017; 10: 617-623.
2. Gaglio R, NC, CM. Evaluation of antimicrobial resistance and virulence of enterococci from equipment surfaces, raw materials, and traditional cheese. International Journal of Food Microbiology. 2016; 236 (2016): 107-114.
3. Hamideh Askari LF, Hamid Reza Pordeli et al. Frequency Distribution of Fluoroquinolones-Resistant Enterococcus faecalis Isolates from Patients with Prostatitis in Golestan Province, Iran. Medical Laboratory Journal. 2019; 13(4): 29-33.
4. Tram QA, và cs. Nghiên cứu đặc điểm kháng kháng sinh của các chủng vi khuẩn Gam dương gây nhiễm khuẩn tiết niệu tại Bệnh viện Hữu nghị đa khoa Nghệ An. Tạp chí Y học Việt Nam. 2022; Số 1: 257-261.
5. Oyamada Y, H. Ito, M. Inoue, Yamagishi. aJ. Topoisomerase mutations and efflux are associated with fluoroquinolone resistance in Enterococcus faecalis. J Med Microbiol. 2006; 55: 1395-1401.
6. Kim MC, and G. J. Woo. Characterization of antimicrobial resistance and quinolone resistance factors in high-level ciprofloxacin-resistant Enterococcus faecalis and Enterococcus faecium isolates obtained from fresh produce and fecal samples of patients. J Sci Food Agric. 2016; 97: 2858-2864.
7. HC Kuo, CC Chous, CD Chang, al e. Characterization of Quinolone-Resistant Enterococcus faecalis Isolates from Healthy Chickens and Pigs in Taiwan. Journal of Food and Drug Analysis. 2009; 17(6): 443-450.
8. Esfahani S, Ahmadrajabi R, al HMe. Co-Incidence of Type II Topoisomerase Mutations and Efflux Expression in High Fluoroquinolone Resistant Enterococcus faecalis Isolated from Urinary Tract Infection. Infection and Drug Resistance. 2020; 13: 553-559.
9. CLSI. M100-ED31:2021 Performance Standards for Antimicrobial Susceptibility Testing, 31st Edition. 2021; 31.
10. Demirgül F, Tuncer Y. Detection of Antibiotic Resistance and Resistance Genes in Enterococci Isolated from Sucuk, a Traditional Turkish Dry-Fermented Sausage. Korean J Food Sci Anim Resou. 2017; 37(5): 670-681.
11. Jia W, Li G, Wang W. Prevalence and Antimicrobial Resistance of Enterococcus Species: A Hospital-Based Study in China. International journal of environmental research and public health. 2014; 11(3): 3424-3442.
12. Wang Y LY, Cai J, et al. A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin.. J Antimicrob Chemother. 2015; 70(8): 2182-2190.
13. Jennifer K. Bender, Carola Fleige, Ingo Klare, Werner G. Development of a multiplex-PCR to simultaneously detect acquired linezolid resistance genes cfr, optrA and poxtA in enterococci of clinical origin. Journal of Microbiological Methods. 2019; 160: 101-103.
14. Corinna Kehrenberg, Schwarz S. Florfenicol-Chloramphenicol Exporter Gene fexA Is Part of the Novel Transposon Tn558. ASM Journals/Antimicrobial Agents and Chemotherapy. 2005; 49: 813-815.
15. Fakhri Haghi, Vahid Lohrasbi, Zeighami H. High incidence of virulence determinants, aminoglycoside and vancomycin resistance in enterococci isolated from hospitalized patients in Northwest Iran. BMC Infectious Diseases. 2019; 19: 744.
16. WHO. 19th WHO model list of essential medicines (April 2015). 2015; WHO, Geneva.
17. Jochen Schulz, Nicole Kemper, Hartung J, al e. Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli. Scientific Reports 2019; 9: 5117.
18. Jiyeun Kate Kim, Ki Yup Nam, In Young Chung, elat. Emerging Enterococcus isolates in postoperative endophthalmitis by selection pressure of fluoroquinolones: An 11-year multicenter and experimental study. Emerging Microbes & Infections 2020; 9(1): 1892-1899.
19. KIM YB, SEO HJ, SEO KW, et al. Characteristics of High-Level Ciprofloxacin-Resistant Enterococcus faecalis and Enterococcus faecium from Retail Chicken Meat in Korea. Journal of Food Protection. 2018; 81(8): 157-1363.
20. Tanih GN. Genotypic and Phenotypic characterization of enterococci from cow dung and environmental water sources in three selected dairy farms in Amathole District. University of Fort Hare, South Africa. 2016;
21. N. Ünal ŞA, and M. Yildirim. Antibiotic resistance profile of Enterococcus faecium and Enterococcus faecalis isolated from broiler cloacal samples. Turkish Journal of Veterinary & Animal Sciences. 2017; 41(2): 199-203.
22. He T SY, Schwarz S, et al. Genetic environment of the transferable oxazolidinone/phenicol resistance gene optrA in Enterococcus faecalis isolates of human and animal origin. . J Antimicrob Chemother. 2016; 71: 1466-1473.
23. Young-Hee Jung, Min-Hyeok Cha, Gun-Jo Woo, Chi. Y-M. Characterization of oxazolidinone and phenicol resistance genes in non-clinical enterococcal isolates from Korea. Journal of global antimicrobial resistance. 2021; 24: 363-368.
24. sSu R, Peng Y, Wang Z, Wu HYaQ. Identification of two novel type II topoisomerase mutations in Enterococcus spp. isolated from a hospital in China. Arch Biol Sci. 2021; 73(3): 407-414.