12. Ovarian tissue cryopreservation by vitrification method: The first experience in Vietnam
Main Article Content
Abstract
Ovarian Tissue Cryopreservation (OTC) is a promising technique for reproductive preservation in women with cancer. Although this technique was applied successfully in many Invitro Fertilization (IVF) centers worldwide, OTC has not been developed in Viet Nam as well as there is no publication of OTC in Vietnam. The study of OTC by vitrification at IVFMD, My Duc Hospital was conducted from 2020 to 2023 with trial registration number NCT04666376. Ovarian tissues from each patient were divided into 3 groups, in which group 1 was fresh tissues as a control group, group 2 was vitrified tissues by Ova kit Type M (Kitazato, Japan) and group 3 was vitrified tissues by IVFMD medium. The findings showed the rates of viable follicles and follicles with both healthy oocyte and CGs in group 2 and 3 were significantly lower than in group 1, at [80.3% in group 1, 70% in group 2 and 65% in group 3] and [71.3% in group 1, 59.4% in group 2 and 58.3% in group 3] respectively (p < 0.05). The results aligned with previous findings because of the negative effects of cryopreservation. Moreover, it was illustrated that there was no change in the quality of tissue between the commercial medium and IVFMD’s medium in OTC.
Article Details
Keywords
Ovarian tissue cryopresvertion, OTC, vitrification
References
2. Rosendahl M, Schmidt KT, Ernst E, et al. Cryopreservation of ovarian tissue for a decade in Denmark: a view of technique. Reprod Biomed Online. 2015;22:162-171. https://doi.or g/10.1016/j.rbmo.2010.10.015
3. Radwan P, Abramik A, Wilczyński J, et al. Successful autotransplan - tation of cryopreserved ovarian tissue with recovery of the ovarian function. Ginekol Pol. 2016;87:235-240. https://doi.org/10.17772/gp/61981
4. Kristensen SG, Rasmussen A, Byskov AG, et al. Isolation of pre-antral follicles from human ovarian medulla tissue. Hum Reprod. 2011;26:157-66. https://doi.org/10.109 3/humrep/deq318
5. Kristensen SG, Liu Q, Mamsen LS, et al. A simple method to quantify follicle survical in cryopreservation human ovarian tissue. Hum reprod. 2018;33(12):2276-2284.
6. Chang HJ, Moon JH, Lee JR, et al. Optimal condition of vitrification method for cryopreservation of human ovarian cortical tis - sues. J Obstet Gynaecol Res. 2011;37:1092-1101.
7. Fabbri R, Vicenti R, Macciocca M, et al. Morphological, ultra- structural and functional imaging of frozen/thawed and vitrified/warmed human ovarian tissue retrieved from oncological patients. Hum Reprod. 2016;31:1838-1849.
8. Langbeen A, Jorssen EPA, Granata N, et al. Effects of neutral red assisted viability assessment on the cryotolerance of isolated bovine preantral follicles. J Assist Reprod Genet. 2014;31(12):1727-1736.
9. Kagawa N, Silber S, Kuwayama M. Successful vitrification of bovine and human ovarian tissue. RBM Online. 2009;18(4):568-577.
10. Donnez J, Dolmans MM. Ovarian cortex transplantation: 60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice. J Assist Reprod Genet. 2015;32:1167-1170.
11. Lan NV, Vinh QD, Tuong MH, et al. IVF transfer of fresh or Frozen Embryos in Women without Polycystic Ovaries. NEJM. 2018;378(2):137-147. doi: 10.1056/NEJMoa1703768.
12. Kitazato. Ova Cryo Kit Protocol - Ovarian tissue vitrification method. https://www.kitazato.co.jp/en/pdf/vitrification/Protocol-EN-OvaCryoKit_210601ver2.pdf.
13. Wang T, Yan J, Lu C, et al. human single follicle growth in invitro from cryopreserved ovarian tisssue after slow freezing or vitrification. Hum Reprod. 2016;31(4):763-773.
14. Isachenko V, Lapidus I, Isachenko E, et al. Human ovarian tissue vitrification ver- sus conventional freezing: morphological, endocrinological, and molecular biological evaluation. Reproduction. 2009;138:319-327.
15. Campos ALM, Guedes JS, Rodrigues JK, et al. Comparison between slow freezing and vitrification in terms of ovarian tissue viability in a bovine model. Rev Bras Ginecol Obstet. 2016;38:333-339.
16. Ramezani M, Salehnia M, Jafarabadi M. Short term culture of vitrified human ovarian cortical tissue to assess the cryopreservation outcome: molecular and morphology analysis. J reprod Infertil. 2017;18(1):162-171.