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Published: 2024-02-27
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DOI: https://doi.org/10.52852/tcncyh.v174i1.2167
Issue
Vol. 174 No. 1 (2024)
Section
Các bài báo
How to Cite
Lan, N. T. T. ., Hà, T. N. T. ., Mai, M. P. Q. ., Trí, N. C. ., Diễm, N. T. N. ., Thư, T. B. ., Kiệt, T. Đình ., Vinh, Đặng Q. ., Tiến, N. V. ., & Tường, H. M. . (2024). 3. Using gene expression as a method to quantify follicle survival in ovarian tissue cryopreservation. Journal of Medical Research, 174(1), 16-26. https://doi.org/10.52852/tcncyh.v174i1.2167

3. Using gene expression as a method to quantify follicle survival in ovarian tissue cryopreservation

Nguyen Thi Thu Lan, Trương Nguyen Thai Ha, Ma Pham Que Mai, Nguyen Cao Tri, Nguyen Thi Ngoc Diem, Tran Bich Thu, Truong Dinh Kiet, Dang Quang Vinh, Nguyen Van Tien, Ho Manh Tuong

Main Article Content

Abstract

Ovarian tissue cryopreservation (OTC) is a promising technique for reproductive preservation in women with cancer. Although this technique was applied successfully in many Invitro Fertilization (IVF) centers worldwide, OTC has not been developed recently, and there is no publication of OTC in Vietnam. Using the expression of GDF-9 and caspase-3 as a biomarker to evaluate the survival of follicles in OTC in IVFMD at My Duc Hospital (NCT04666376) is the first study conducted in Vietnam. Ovarian tissues from each donor were divided into 3 groups, which group 1 was fresh tissues as a control group, group 2 was vitrified tissues by Ova kit Type M (Kitazato, Japan) and group 3 was vitrified tissues by IVFMD medium. Then, mRNA was extracted and transcripted reversely to cDNA before realtime PCR was performed. The findings showed there was no statistical difference between 3 groups about the expression of GDF-9 and caspase-3, respectively, [0.66, 1.38 in group 1 vs group 2] and [0.71, 1.08 in group 1 vs group 3], p > 0.05. The results aligned with previous findings because of the negative effects of cryopreservation. Moreover, it was illustrated that there was no change in tissue quality between the commercial medium and IVFMD’s medium in OTC.

Article Details

Keywords

Ovarian tissue cryopreservation, OTC, vitrification, GDF-9, caspase-3

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