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Published: 2024-03-29
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DOI: https://doi.org/10.52852/tcncyh.v175i2.2232
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Vol. 175 No. 2 (2024)
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Các bài báo
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Hạnh, N. T. H., Mão, C. V., & Cường, B. K. (2024). 20. Survey of TNF-α concentration and its correlation with leukocyte indicators in mice treated with anti-PD-1 monoclonal antibody and CAR-T cells combination. Journal of Medical Research, 175(2), 164-171. https://doi.org/10.52852/tcncyh.v175i2.2232

20. Survey of TNF-α concentration and its correlation with leukocyte indicators in mice treated with anti-PD-1 monoclonal antibody and CAR-T cells combination

Nguyen Thi Hien Hanh, Can Van Mao, Bui Khac Cuong

Main Article Content

Abstract

The purpose of the study is to evaluate the correlation between leukocyte indices and TNF-α levels of combination therapy of monoclonal antibody that inhibits PD-1 with CAR-T cells in the experimental animal models. We conducted an experimental, prospective, interventional study with a control group. 60 white mice were divided into 4 groups, each group of 15 animals including a control group, CAR-T cell treatment group, PD-1 inhibitor monoclonal antibody treatment group, and PD-1 combined with CAR-T cells group. After treatment, all mice were monitored for whole body condition. Their white blood cell indices and TNF-α levels were measured. TNF-α concentration in the CAR-T group was not different from the control group. TNF-α levels in the PD-1 inhibitor monoclonal antibody combined with CAR-T cells increased statistically significantly compared to the control group (p < 0.001). There is a positive correlation between TNF-α concentration and the absolute number of monocytes in the treatment group of monoclonal antibody that inhibits PD-1 combined with CAR-T cells (r = 0.578; p < 0.05 ). Through this research, we found that combining anti-PD-1 monoclonal antibodies with CAR-T cells increased TNF-α levels in mice and there was a positive correlation between TNF-α concentration and monocyte count.

Article Details

Keywords

CAR-T, PD-1, white blood cell indices, TNF-α

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