Nghiên cứu tạo mô hình chuột mang khối u ghép bằng tế bào HT29 Hyper-IL-6

Abstract

The IL-6/STAT3 signaling pathway plays a critical role in the progression of colorectal cancer (CRC). Selective inhibition of IL-6 trans-signaling is a promising therapeutic approach; however, appropriate preclinical models for evaluation remain limited. Therefore, we established a mouse model bearing colorectal tumors with enhanced IL-6 expression, providing a platform to investigate the biological functions of IL-6 signaling. A xenograft model was generated in BALB/c nude mice by subcutaneous injection of 3x10⁶ HT29 hyper IL-6 cells into the dorsal flank. Tumor size was monitored every three days. At study endpoint, tumor tissues were analyzed by histopathology (H&E) and immunohistochemistry (p40, CDX-2). Palpable tumors formed within 4 days, with consistent tumor growth and size doubling after 3 days. IHC showed p40 negativity and CDX-2 positivity, confirming colorectal glandular epithelial origin and the model’s relevance to human CRC. In conclusion, this study successfully established a nude mouse xenograft model with hyper IL-6 colorectal tumors. Histopathological and immunohistochemical findings validated the formation of characteristic colorectal adenocarcinoma tumors.