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Published: 2023-01-10
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DOI: https://doi.org/10.52852/tcncyh.v160i12V1.1149
Issue
Vol. 160 No. 12V1 (2022)
Section
Các bài báo
How to Cite
Đạt, N. X. ., Hồng, V. T. B. ., Hiếu, P. V. ., Hà, N. T. ., Hưng, L. V. ., & Dung, Đặng T. N. . (2023). 2. Developing procedure for producing external quality assessment panel for fungi. Journal of Medical Research, 160(12V1), 8-17. https://doi.org/10.52852/tcncyh.v160i12V1.1149

2. Developing procedure for producing external quality assessment panel for fungi

Nguyen Xuan Dat, Vu Thi Bich Hong, Phan Van Hieu, Nguyen Thuy Ha, Le Van Hung, Dang Thi Ngoc Dung

Main Article Content

Abstract

Optimizing the technology and process for producing external quality assessment (EQA) panel is an important requirement for an EQA provider. The procedure for producing EQA panel for fungal staining, fungal identification, and antifungal biotics check was studied on 4 fungal strains, isolated from patients and cultured from standard strains: Candida albicans, Cryptococcus neoformans, Candida tropicalis, and Candida auris. The lyophilizing process included freezing stage at -40oC, primary drying at -33oC, secondary drying at 25oC, pressure at 200mtor, on 10% lactose substrate using Virtis Advantage Pro SP Scientific instrument. The lyophilized products had required criteria as survival > 103 CFU/ml, residual moisture < 4%, homogeneity at 100%, and stable for at least 8 months. For the liquid samples set: the morphology of Cryptococcus neoformans remained stable for 32 days, the morphology of Candida albicans, Candida tropicalis and Candida auris remained stabled for at least 40 days.

Article Details

Keywords

fungals, external quality assessment

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